Skip to main content
SpringerLink
Log in

The metabolism of indoleacetic acid by barley grains

  • Published:
Planta Aims and scope Submit manuscript

Summary

It has been shown that indoleacetic acid (IAA) does not occur in developing grains of Hordeum vulgare L. (barley), but that an unidentified indolic compound does. This compound, designated ‘A’, was also found to be a product of the metabolism of exogenous IAA by barley. The expression of the gibberellic acid effect was delayed for at least 8 h if grains were imbibed in a solution of IAA, and during this time, the IAA was metabolised. The enzyme system involved could be peroxidase, which was active in the grains at all stages of their development and at maturity, and partially purified extracts of peroxidase were found to have considerable IAA oxidase activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DMABA:

dimethyl aminobenzaldehyde

dMACA:

Dimethyl amino cinnamylaldehyde

GA:

gibberellic acid

IAA:

indole acetic acid

References

  • Andreae, W. A., Van Ysselstein, M. W. H.: Studies on indole acetic acid metabolism. III. The uptake of IAA by pea epicotyls and its conversion to indoleacetylaspartate. Plant Physiol. 31, 235–249 (1956)

    Google Scholar 

  • Bandurski, R. S., Ueda, M., Nicholls, P. B.: Esters of indole acetic acid and myo-inositol. Ann. N. Y. Acad. Sci. 165, 655–667 (1969)

    Google Scholar 

  • Briggs, D. E.: α-amylase in germinating decorticated barley. Phytochem. 7, 513–529 (1968)

    Google Scholar 

  • Davis, B. J.: Disc Electrophoresis. II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121, 404–427 (1964)

    Google Scholar 

  • Endo, T.: Indole acetic acid oxidase activity in horseradish and other plant peroxidase isoenzymes. Pl. and Cell Physiol. 9, 333–341 (1968)

    Google Scholar 

  • Good, N. E., Andreae, W. A., Van Ysselstein, M. W. H.: Studies on indole acetic acid metabolism. II. Some products of the metabolism of exogenous indole acetic acid in plant tissues. Plant Physiol. 31, 231–235 (1956)

    Google Scholar 

  • Harmey, M. A., Murray, A.: The effect of gibberellic acid on peroxidase levels in barley. Planta (Berl.) 83, 387–389 (1968)

    Google Scholar 

  • Krupasager, V., Sequeira, L.: Auxin destruction by Alarasmius perniciosus. Amer. J. Bot. 56, 390–397 (1969)

    Google Scholar 

  • MacLeod, A. M., Duffus, J. H., Johnston, C. S.: Development of hydrolytic enzymes in germinating grain. J. Inst. Brew. 70, 521–528 (1964)

    Google Scholar 

  • Meudt, W. J., Powell-Gaines, T.: Studies on the oxidation of indole acetic acid by peroxidase enzymes. Pl. Physiol. 42, 1395–1399 (1967)

    Google Scholar 

  • Mino, Y.: Studies on the destruction of indole acetic acid by a species of Arthrobactor. IV. Decomposition products. Plant and Cell Physiol. 11, 128–138 (1970)

    Google Scholar 

  • Mollan, R. C., Donnelly, D. M. X., Harmey, M. A.: Synthesis of indole-3-acetylaspartic acid. Phytochem. 11, 1485–1488 (1972)

    Google Scholar 

  • Murray, A., Harmey, M. A.: Peroxidase in barley endosperm as a bioassay for gibberellins. Sci. Proc. roy. Dublin Soc. 2, 275–281 (1970)

    Google Scholar 

  • Parish, H. W.: Studies on senescing tobacco leaf dises with special reference to peroxidase. Planta (Berl.) 82, 1–21 (1968)

    Google Scholar 

  • Pollard, C. J.: A survey of the sequence of some effects of gibberellic acid in the metabolism of cereal grains. Plant Physiol. 44, 1227–1232 (1969)

    Google Scholar 

  • Shannon, L. M., Kay, S., Lew, J. Y.: Peroxidase isoenzymes from horseradish roots. J. biol. Chem. 241, 2166–2172 (1966)

    Google Scholar 

  • Shrivastava, B. I. S.: Ether soluble and ether insoluble auxins from immature corn kernals. Plant Physiol. 38, 473–478 (1963)

    Google Scholar 

  • Stahl, E.: Thin-layer chromatography, a laboratory handbook, Berlin-Heidelberg-New York: Springer 1965

    Google Scholar 

  • Tafuri, F.: IAA determination in the kernals of four lines of corn and of their hybrids. Phytochem. 5, 999–1013 (1966)

    Google Scholar 

  • Ueda, M., Bandurski, R. S.: A quantitative estimation of alkali-labile IAA compounds in dormant and germinating maize kernals. Plant Physiol. 44, 1175–1181 (1969)

    Google Scholar 

  • Varner, J. E., Ram Chandra, G.: Hormonal control of enzyme synthesis in barley endosperm. Proc. nat. Acad. Sci. (Wash.) 52, 100–106 (1964)

    Google Scholar 

  • Veen, H.: Naphthylacetic acid-1-14C in Coleus extracts. Acta bot. neerl. 15, 419–433 (1966)

    Google Scholar 

  • Zenk, M. H.: 1-(Indole-3-acetyl)-D-glucose, a new compound in the metabolism of indole acetic acid in plants. Nature (Lond.) 191, 165–177 (1961)

    Google Scholar 

Download references

Author information

Author notes

  1. Ann Minchin

    Present address: Department of Agriculture, University of Reading, Earley Gate, RG6 2AT, Reading, UK

Authors and Affiliations

  1. Department of Botany, University College, Belfield, Stillorgan Road, Dublin 4, Eire

    Ann Minchin & Matthew A. Harmey

Authors
  1. Ann Minchin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Matthew A. Harmey
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Minchin, A., Harmey, M.A. The metabolism of indoleacetic acid by barley grains. Planta 122, 245–254 (1975). https://doi.org/10.1007/BF00385272

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00385272

Keywords

Search

Navigation