Summary
The contribution of individual leaves towards the flowering response in red clover (Trifolium pratense L.) seedlings at the three-leaf stage is described. Removal of the first (oldest) or first and second leaves resulted in large increases (up to 300%) in both the rate of stem extension and the degree of apical differentiation.
Removal of the youngest leaves depressed both processes. Application of cycloheximide to specific leaves produced effects similar to defoliation, but chloramphenicol was generally inhibitory and kinetin substantially ineffective. Translocation patterns between leaves and the shoot apex were studied using [14C] sucrose and [3H] gibberellin A1. There was appreciable movement between leaves, but exposure to long-days depressed the transport of labelled assimilate. Label arriving at the apex was increasingly derived from the younger leaves as floral induction proceeded.
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Abbreviations
- CAP:
-
D-threo-chloramphenicol
- CH:
-
cycloheximide
- K:
-
Kinetin
- Tris:
-
Tris(hydroxymethyl) amino methane
- G.S.H.:
-
Glutathione (reduced)
- E.D.T.A.:
-
ethylene diamino tetra acetic acid disodium salt
References
Chin, T., Beevers, L.: Changes in endogenous growth regulators in Nasturtium leaves during senescence. Planta (Berl.) 92, 178–188 (1970).
Ellis, R. J., MacDonald, I. R.: Specificity of cycloheximide in higher plant systems. Plant Physiol. 46, 227–232 (1970).
Evans, L. T.: Inflorescence initiation in Lolium temulentum L. 1. Effect of plant age and leaf area on sensitivity to photoperiodic induction. Aust. J. biol. Sci. 13, 123–131 (1960).
Hedley, C. L., Stoddart, J. L.: Factors influencing alanine aminotransferase activity in leaves of Lolium temulentum L. 1. Photoperiodically induced variations. J. exp. Bot. 22, 239–248 (1971a).
Hedley, C. L., Stoddart, J. L.: Light stimulation of alanine aminotransferase activity in dark-grown leaves of Lolium temulentum L. as related to chlorophyll formation. Planta (Berl.) 100, 309–324 (1971b).
Hedley, C. L., Stoddart, J. L.: Patterns of protein synthesis in Lolium temulentum L. J. exp. Bot. 23, 490–501 (1972)
Jones, R. L., Phillips, I. D. J.: Organs of gibberellin synthesis in light-grown sunflower. Plant Physiol. 41, 1381–1386 (1966).
Jones, T. W. A., Stoddart, J. L.: Gibberellin-induced changes in protein synthesis and enzyme activity in shoot apices of Trifolium pratense. J. exp. Bot. 21, 453–461 (1970).
Kende, H. J.: Preparation of radioactive gibberellin A1 and its metabolism in dwarf peas. Plant Physiol. 42, 1612–1618 (1967).
Khudairi, A. K., Hamner, K. C.: The relative sensitivity of Xanthium leaves of different ages to photoperiodic induction. Plant Physiol. 29, 251–257 (1964).
King, R. W., Zeevaart, J. A. D.: Characteristics of floral stimulus movement in Perilla and the nature of flower inhibition caused by non-induced leaves. Plant Research 71. Rep. MSU/AEC Plant Research Lab. East Lansing; p. 51–57 (1972).
Lang, A.: Physiology of flower initiation. In: Encyclop. of plant physiol. vol. XV/1, p. 1380–1536. Berlin-Heidelberg-New York: Springer 1965.
Sailsbury, F. B.: The dual role of auxin in flowering. Plant Physiol. 30, 327–334 (1955).
Wareing, P. F., Khalifa, M. M., Treharne, K. J.: Rate limiting processes in photosynthesis at saturating light intensities. Nature (Lond.) 220, 453–457 (1968).
Zeevaart, J. A. D.: Flower formation as studied by grafting. Meded. Langbouwhoogeschool Wageningen 58 No 3 (1958).
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Jones, T.W.A., Stoddart, J.L. Correlative effects of leaf age on reproductive growth in red clover (Trifolium pratense L.). Planta 113, 67–77 (1973). https://doi.org/10.1007/BF00385190
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DOI: https://doi.org/10.1007/BF00385190