Summary
By ultrastructural methods, cytology, and cytochemistry it is shown that peroxysomes are present during all stages of the life cycle of the green unicellular alga Micrasterias fimbriata, cultivated on mineral medium. These organelles, surrounded by a single membrane, are in connection with endoplasmic reticulum. In full-grown cells, they are preferentially situated near chloroplasts and cell walls. The number of peroxisomes increase before cellular division and the organelles flow into the young bulge in front of the chloroplast.
Application of a modified Graham and Karnosky's medium using DAB at pH 9 shows that an important activity of catalase is present not only at the level of peroxisomes but also at the level of the cell walls and certain Golgi vesicles.
The topographic relations of peroxisomes with different cellular organelles and their possible functions in cell wall or mucus synthesis are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Czaninski, Y., Catesson, A. M.: Localisation ultrastructurale d'activités peroxydasiques dans les tissus conducteurs végétaux en cours du cycle annuel. J. Micr. 8, 875–888 (1969).
DeDuve, C., Baudhuin, P.: Peroxysomes (microbodies and related particles). Physiol. Rev. 46, 323–357 (1966).
Drumm, H. Falk, H., Möller, J., Mohr, H.: The development of catalase in the mustand seedlings. Cytobiol. 2, 335–340 (1970).
Frederick, S. E., Newcomb, E. H.: Cytochemical localization of catalase in leaf microbodies (Peroxysomes). J. Cell Biol. 43, 343–353 (1969).
Gerhardt, B., Berger, Ch.: Microbodies and diaminobenzidin Reaktion in den Acetat-Flagellaten Polytomella caeca und Chlorogonium elongatum. Planta (Berl.) 100, 155–156 (1971).
Gouhier, M., Tourte, M.: Nature et rôle de quelques formations particulières chez Micrasterias fimbriata (Ralfs). J. Micr. 8, 50a (1969).
Graham, R. C., Karnosky, M. J.: The early stages of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291 (1966).
Graves, L. B., Jr., Hanzely, L., Trelease, R. N.: The occurence and fine structural characterisation of microbodies in Euglena gracilis. Protoplasma 72, 141–152 (1971).
Hilliard, J. H., Gracen, V. E., West, S. H.: Leaf mictobodies (Peroxysomes) and catalase localization in plants differing in their photosynthetic carbon pathways. Planta (Berl.) 97, 93–105 (1971).
Kiermayer, O.: Elektronenmikroskopische Untersuchungen zum Problem der Cytomorphogenese von Micrasterias denticulata Breb. I. Allgemeiner Überblick. Protoplasma 69, 97–132 (1970).
Marty, D.: Peroxysomes (Microbodies) associés aux plastes des zones non chlorophyllinnes des feuilles panachées de Coleus blumei Benth. C. R. Acad. Sci. (Paris), sér. D 273, 860–863 (1971).
Marty, F.: Caractérisation cytochimique infrastructurale de peroxisomes (“microbodies” sensu stricto) chez Euphorbia characias. C. R. Acad. Sci. (Paris), sér. D 268, 1388–1391 (1969).
Marty, F.: Les peroxysomes (microbodies) des lactifères d'Eurphorbia characias L. Une étude morphologique et cytochimique. J. Micr. 9, 923–948 (1970).
Mollenhauer, H. H., Moore, D. J., Kelley, A. G.: The widespread occurence of plant cytosomes resembling animal microbodies. Protoplasma 62, 44–52 (1966).
Novikoff, A. B., Goldfischer, S.: Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine. J. Histochem. Cytochem. 17, 675–680 (1969).
Poux, N.: Localisation d'activités enzymatiques dans les cellules du méristème radiculaire de Cucumis sativus L. II. Activité péroxydasique. J. Micr. 8, 855–866 (1969).
Poux, N.: Réactions avec le DAB dans le limbe foliaire de Dactylis glomerata L. (graminées). Mise en évidence de Peroxysomes. J. Micr. 11, 87–88 (1971).
Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. cell Biol. 17, 208–213 (1963).
Vigil, E. L.: Cytochemical and developmental changes in microbodies (glyoxysomes) and related organelles of castor bean endosperm. J. Cell Biol. 46, 453–454 (1970).
Tolbert, N, E., Yamazaki, R. K.: Leaf peroxisomes and their relations to photorespiration and photosynthesis. Ann. N. Y. Acad. Sci. 168, 321–341 (1969).
Tourte, M.: Identification et ròle des vésicules de type “peroxysome” chez Micrasterias fimbriata. J. Micr. 11, 96 (1971).
Wood, R. L., Legg, P. G.: Peroxidase activity in Rat liver microbodies after amino-triazole inhibition. J. Cell Biol. 45, 576–585 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tourte, M. Mise en evidence d'une activité catalasique dans les peroxysomes de Micrasterias fimbriata (Ralfs). Planta 105, 50–59 (1972). https://doi.org/10.1007/BF00385163
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00385163