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The ability of amino compounds and conditioned medium to alleviate the reduced nitrogen requirement of soybean cells grown in suspension cultures

Planta volume 105, pages 25–32 (1972)Cite this article

Summary

Various nitrogen compounds were tested for their ability to alleviate the reduced nitrogen requirement of soybean cells growing in defined liquid medium containing nitrate as the alternative nitrogen source. Either l-glutamine, l-alanine, putrescine or NH4 + satisfied this requirement. Addition of l-glutamate resulted in poor growth. Where growth was stimulated, nitrate reductase (NR) activity increased whereas glutamate dehydrogenase activity in the cells showed no such correlation. In all fresh media which supported rapid growth, NR activity first decreased rapidly to a low value. Subsequent dry weight increases occurred concommitantly with an increase in NR activity. When 2,4-dichlorophenoxyacetic acid was omitted from the medium the growth was slow and the NR activity did not increase. During the first 40 h of incubation in medium containing NH4 + plus NO3 - the cells produced a growth-enhancing factor(s). This factor(s) was present in the cells and in the medium and eliminated the requirement for reduced nitrogen.

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Author notes

  1. J. M. Bayley

    Present address: , 34 Favvel Road, Glossop, Derbyshire, England

Affiliations

  1. Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    J. M. Bayley, J. King & O. L. Gamborg

  2. Prairie Regional Laboratory, National Research Council of Canada, Saskatoon, Saskatchewan, Canada

    J. M. Bayley, J. King & O. L. Gamborg

Authors
  1. J. M. Bayley
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  2. J. King
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  3. O. L. Gamborg
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Additional information

This work was supported by a grant in aid of research from the National Research Council of Canada to one of us (J. K.). NRCC No. 12520

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Bayley, J.M., King, J. & Gamborg, O.L. The ability of amino compounds and conditioned medium to alleviate the reduced nitrogen requirement of soybean cells grown in suspension cultures. Planta 105, 25–32 (1972). https://doi.org/10.1007/BF00385160

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  • DOI: https://doi.org/10.1007/BF00385160

Keywords

  • Glutamate
  • Nitrogen Source
  • Conditioned Medium
  • Suspension Culture
  • Nitrate Reductase