Abstract
Mature pollen grains of Trachymene pilosa (Umbelliferae) were treated at 40° C in 2% aqueous osmium for one to six days. Exine observed by (TEM) were progressively destroyed. After six days, they were reduced to a three-dimensional structure composed only of pollenkitt.
This is a preview of subscription content, log in via an institution to check access.
References
Brook J., Elsik W.C. (1974) Chemical oxidation (using ozone) of the spore wall of Lycopodium clavatum. Grana 14, 85–91
Brook J., Shaw G. (1978) Sporopollenin: a review of its chemistry paleochemistry and geochemistry. Grana 17, 91–97
Chaloner W.G. (1976) The evolution of adaptive features in fossil exines. In: The evolutionary significance of the exine, Linnean Society Symposium, sèries no 1, 1–14, Ferguson I.K., Muller J. ed., Academic Press Inc
Larson P.A. (1964) Processing pollen and spores exines for electron microscopy. Stain Technol. 39, 237–243
Poux N. (1973) Observation en microscopie électronique de cellules végétales imprégnées par l'osmium. C.R. Acad. Sci. Paris 276, 2163–2166
Roland J.C., Roland F. (1968) Essais de coloration sélective des niveaux du sporoderme en microscopie électronique. Pollen Spores X, 5–10
Roland-Heydacker F., Cerceau-Larrival M.Th. (1978) Ultrastructure du tectum de pollens d'Ombellifères. Grana 17, 81–89
Rowley J.R., Prijanto B. (1977) Selective destruction of the exine of pollen grain. Geophytology 7, 1–23
Southworth D. (1974) Solubility of pollen exines. Am. J. Bot. 61, 33–44
Thiery J.P. (1967) Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J. Microsc. 6, 987–1018
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Audran, J.C. Degeneration of Trachymene pilosa exine by osmium tetroxide used in impregnation technique. Planta 152, 282–284 (1981). https://doi.org/10.1007/BF00385157
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00385157