Abstract
Using Concanavalin A (Con A) labeled with fluorescein isothiocyanate, we studied the intracellular localization of receptor molecules in the calyptra of 24-h dark-grown cress roots. Fixation in glutaraldehyde gave positive binding of the distal complex of the endoplasmic reticulum and the nucelus in the statocytes. In contrast, fixation in formaldehyde did not preserve the membrane-associated receptors, but revealed Con A affinity of the starch grain surface within the amyloplasts. Treatment of glutaraldehydefixed sections with non-ionic detergents led to partial solubilization of membrane components: the starch grain surface turned positive, though the positive binding of Con A to the endoplasmic reticulum and the nucleus remained unaffected. We therefore conclude that the Con A receptor in the membrane is a glycoprotein tightly inserted in other components of the compartment.
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Abbreviations
- Con A:
-
Concanavalin A
- ER:
-
endoplasmic reticulum
- FITC:
-
fluorescein isothiocyanate
- NP 40:
-
nonidet P40
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Schneider, E.M., Sievers, A. Concanavalin A binds to the endoplasmic reticulum and the starch grain surface of root statocytes. Planta 152, 177–180 (1981). https://doi.org/10.1007/BF00385141
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DOI: https://doi.org/10.1007/BF00385141