Summary
Liberation of 14CO2 from 14C urea by Spirodela oligorrhiza plants was proportional to their level of urease as assayed by formation of ammonia in extracts. The composition of the soluble nitrogen pool of plants growing on urea differed from that of plants growing on ammonium, mainly with regard to levels of amides and arginine. Neither level of urea in the medium nor of ammonia in the tissues appeared to have a major effect on level of urease activity.
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Baker, J. E. and J. F. Thompson: Metabolism of urea and ornithine cycle intermediates by nitrogen-starved cells of Chlorella vulgaris. Plant Physiol. 37, 618–624 (1962).
Bieleski, R. L., and N. A. Turner: Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chromatography. Analyt. Biochem. 17, 278–293 (1966).
Bollard, E. G., A. R. Cook, and N. A. Turner: Urea as sole source of nitrogen for plant growth. I. The development of urease activity in Spirodela oligorrhiza. Planta (Berl.) 83, 1–12 (1968).
Cook, A. R., and R. L. Bieleski: Fractionation of plant extracts by thin-layer chromatographic and electrophoretic procedures. Analyt. Biochem., in press (1968).
—, and D. Boulter: Utilization of urea by Candida flareri. Phytochem. 3, 313–319 (1964).
Gale, G. R.: Inhibition of urease by hydroxyurea. Biochem. Pharmacol. 14, 693–698 (1965).
Hattori, A.: Studies on the metabolism of urea and other nitrogenous compounds in Chlorella ellipsoidea. I. Assimilation of urea and other nitrogenous compounds by nitrogen-starved cells. J. Biochem. 44, 253–273 (1957).
—: Studies on the metabolism of urea and other nitrogenous compounds in Chlorella ellipsoidea. II. Changes on levels of amino acids and amides during the assimilation of ammonia and urea by nitrogen-starved cells. J. Biochem. 45, 57–64 (1958).
König, C., u. H. G. Schlegel: Oscillationen der Ureaseaktivität von Hydrogenomonas H16 in statischer Kultur. Biochim. biophys. Acta (Amst.) 139, 182–185 (1967).
Valentine, R. C., and R. S. Wolfe: Phosphate-dependent degradation of urea. Nature (Lond.) 191, 925–926 (1961).
Yemm, E. W., and E. C. Cocking: The determination of amino acids with ninhydrin. Analyst 80, 209–214 (1955).
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Cook, A.R. Urea as sole source of nitrogen for plant growth. Planta 83, 13–19 (1968). https://doi.org/10.1007/BF00385131
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DOI: https://doi.org/10.1007/BF00385131