Abstract
In etiolated rye seedlings (Secale cereale L.) the cytokinin supply controls leaf growth. Dry weight and total amino-nitrogen, protein, total nucleic acid, DNA, and rRNA levels were similarly lowered in leaves depleted of their endogenous cytokinin supply by early excision of the seedling roots and increased by 70–100% after the addition of kinetin. The proportion of cytoplasmic ribosomes bound as polyribosomes was only slightly increased from 40% in cytokinin-deficient to 50% in kinetin-treated darkgrown leaves. White light increased the polyribosome proportion to 61%. In cytokinin-supplied leaves uptake and accumulation of L-[3H]leucine were greater than in cytokinin-deficient leaves. Under all conditions of cytokinin supply the same proportion of the total amino nitrogen content (80%) was present as protein nitrogen and virtually, the same percentage (60%) of the total uptake of L-[3H]leucine was incorporated into protein in dark-grown leaves. In light, significantly higher proportions of the amino nitrogen content and of the L-[3H]leucine uptake were incorporated into protein. The results suggest that the accumulation of substrate is a main cytokinin-controlled step determining the growth of the leaves.
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Boer, J. de, Feierabend, J.: Comparison of the effects of cytokinins on enzyme development in different cell compartments of the shoot organs of rye seedlings. Z. Pflanzenphysiol 71, 261–270 (1974)
Burrows, W.J.: Mechanism of action of cytokinins Curr. Advances Plant Sci. 21, 837–847 (1975)
Crestfield, A.M., Smith, K.C., Allen, F.W.: The preparation and characterization of ribonucleic acids from yeast. J. Biol. Chem. 216, 185–193 (1955)
Davies, E., Larkins, B.A., Knight, R.H.: Polyribosomes from peas. An improved method for their isolation, in the absence of ribonuclease inhibitors. Plant Physiol. 50, 581–584 (1972)
Feierabend, J.: Der Einfluß von Cytokininen auf die Bildung von Photosyntheseenzymen in Roggenkeimlingen. Planta 84, 11–24 (1969)
Feierabend, J.: Characterization of cytokinin action on enzyme formation during the development of the photosynthetic apparatus in rye seedlings. Planta 94, 1–15 (1970)
Feierabend, J., de Boer, J.: Comparative analysis of the action of cytokinin and light on the formation of ribulosebisphosphate carboxylase and plastid biogenesis. Planta 142, 75–82 (1978)
Feierabend, J., Schrader-Reichhardt, U.: Biochemical differentiation of plastids and other organelles in rye leaves with a hightemperature-induced deficiency of plastid ribosomes. Planta 129, 133–145 (1976)
Fox J.E., Erion, J.L.: A cytokinin binding protein from higher plant ribosomes. Biochem. Biophys. Res. Commun. 64, 694–700 (1975)
Gordon, M.E., Letham, D.S.: Regulators of cell division in plant tissues. XXII. Physiological aspects of cytokinin-induced radish cotyledons growth. Aust. J. Plant Physiol. 2, 129–154 (1975)
Hall, R.H.: Cytokinins as a probe of developmental processes. Ann. Rev. Plant Physiol. 24, 415–444 (1973)
Kende, H.: The cytokinins. Intern. Rev. Cytol. 31, 301–338 (1971)
Klämbt, D.: Cytokinin effects on protein synthesis of in vitro systems of higher plants. Plants & Cell Physiol 17, 73–76 (1976)
Maaß, H., Klämbt, D.: Cytokinin effect on protein synthesis in vivo in higher plants. Planta 133, 117–120 (1977)
Mothes, K.: Über das Altern der Blätter und die Möglichkeit ihrer Wiederverjüngung. Naturwissenschaften 47, 337–351 (1960)
Parish, J.H., Kirby, K.S.: Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochim. Biophys Acta 129, 554–562 (1966)
Paul, D.: Über die Wirkung von Cytokininen auf die Synthese von Proteinen und Nukleinsäuren in etiolierten Primärblättern von Phaseolus vulgaris L. Doctoral Dissertation Universität Göttingen (W.-Germany) (1974)
Pilet, P.E., Braun, R.: Ribonuclease activity and auxin effects in the lens roots. Physiol. Plant. 23, 245–250 (1970)
Ruppel, H.G., van Wyk, D.: Über die Desoxyribonukleinsäure in Chloroplasten von Antirrhinum majus. Z. Pflanzenphysiol. 53, 32–38 (1965)
Schäfers, H.-A., Feierabend J.: Ultrastructural differentiation of plastids and other organelles in rye leaves with a high-temperature-induced deficiency of plastid ribosomes. Cytobiologie 14, 75–90 (1976)
Schmidt, G., Thannhauser, S.J.: A method for the determination of disoxyribonucleic acid and phosphoproteins in animal tissues. J. Biol. Chem. 161, 83–89 (1945)
Shininger, T.L., Powell, L.D.: Biochemical responses of pea root tissue to cytokinin. Enhanced rates of RNA synthesis. Plant Physiol. 59, 831–835 (1977)
Short, K.C., Tepfer, D.A., Fosket, D.E.: Regulation of polyribosome formation and cell division in cultured soybean cells by cytokinin. J. Cell Sci. 15, 75–87 (1974)
Skoog, F., Armstrong, D.J.: Cytokinins. Ann. Rev. Plant Physiol. 21, 359–384 (1970)
Takegami, T., Yoshida, K.: Specific interaction of cytokinin binding protein with 40S ribosomal subunits in the presence of cytokinin in vitro. Plant & Cell Physiol 18, 337–346 (1977)
Torrey, J.G.: Root hormones and plant growth. Ann. Rev. Plant Physiol. 27, 435–459 (1976)
Travis, R.L., Key, J.L.: Correlation between polyribosome level and the ability to induce nitrate ductase in dark-grown corn seedlings. Plant Physiol. 48, 617–620 (1971)
Varner J.E., Chandra, G.R.: Hormonal control of enzyme synthesis in barley endosperm. Proc. Natl. Acad. Sci. USA 52, 100–106 (1964)
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de Boer, J., Feierabend, J. Comparative analysis of the action of cytokinin and light on the growth of rye leaves. Planta 142, 67–73 (1978). https://doi.org/10.1007/BF00385122
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DOI: https://doi.org/10.1007/BF00385122