Abstract
The effect of gibberellic acid on the secretion of proteins from barley (Hordeum vulgare L.) aleurone layers has been investigated for its suitability as a gibberellin bioassay. Concentrations from 10−4 μg/ml to 100 μg/ml of GA3 resulted in the release of proportionally increasing amounts of total protein. The release of proteins is not affected by indoleacetic acid and kinetin. This method has been applied and compared with the α-amylase assay for the estimation of gibberellin in extracts of tomato fruits and maize seedlings.
Similar content being viewed by others
Abbreviations
- GA3 :
-
gibberellic acid
- IAA:
-
indoleactic acid
- K:
-
kinetin
References
Abdel-Rahman, M., Thomas, T.H., Doss, G.J., Hawell, L.: Changes in endogenous plant hormones in cherry tomato fruits during development and maturation. Physiol. Plant. 34, 39–43 (1975)
Ashworth, U.S.: Proteins in meat egg products determined by dye binding. J. Food Sci. 36, 509–510 (1971)
Audus, L.J.: The gibberellins. In: Plant growth substances, pp. 104–131. London: Leonard Hill 1972
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254 (1976)
Briggs, D.E.: Biochemistry of barley germination: Action of gibberellic acid on barley endosperm. J. Inst. Brew. 69, 13–19 (1963)
Chrispeels, M.J., Varner, J.E.: Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol 42, 398–406 (1967)
Crozier, A., Kuo, C.C., Durley, R.C., Pharis, R.P.: The biological activities of 26 gibberellins in nine plant bioassays. Can. J. Bot. 48, 867–877 (1970)
Harmey, M.A., Murray, A.M.: The effect of gibberellic acid on peroxidase levels in barley. Planta 83, 387–389 (1968)
Jones, R.L., Varner, J.E.: The bioassay of gibberellins. Planta 72, 155–161 (1967)
Mapelli, S., Frova, C., Torti, G., Soressi, G.P.: Relative level of endogenous plant hormones in parthenocarpic tomato mutant during development. In: 9th Int. Conf. Plant Growth Substances, Collected Abstracts, Pilet, P.E., ed. pp. 238–239, Lausanne 1976
Melcher, U., Varner, J.E.: Protein release by barley aleurone layers. J. Inst. Brew. 77, 456–461 (1971)
Moss, U.G., Kielsmeier, E.W.: A method for rapid determination of protein in meat by dye binding. Food Technol 21, 351–354 (1967)
Murphy, J.B., Kies, M.W.: Note on spectrophotometric determination of prtein in dilute solution. Biochim. Biophys. Acta 45, 382–384 (1960)
Nicholls, P.B., Paleg, L.G.: A barley endosperm bioassay for gibberellins. Nature 199, 823–824 (1963)
Paleg, L.G.: Physiological effects of gibberellic acid. I. On carbohydrate metabolism and amylase activity of barley endosperm. Plant Physiol. 35, 293–299 (1960a)
Paleg, L.G.: Physiological effects of gibberellic acid. II. On starchhydrolyzing enzymes of barley endosperm. Plant Physiol. 35, 902–906 (1960b)
Redei, G.: Triticum durum abyssinicum x Secale cereale by hybridek elöallitosa measterséges embryo nevelés segitségerel. Novenytermeles 4, 365–367 (1955)
Taiz, L., Jones, R.L.: Gibberellic acid, β-1,3 glucanase and the cell walls of barley aleurone layers. Planta 92, 73–84 (1970)
Varner, J.E.: Gibberellic acid controlled synthesis of α-amylase in barley endosperm. Plant Physiol. 39, 413–415 (1964)
Yomo, H.: Studies on the amylase activating substances. IV. On the amylase activating action of gibberellin. Hakko Kyokaishi 18, 600–602 (1960) (cit. in) Chem. Abstr. 55, 26145 (1961)
Yomo, H.: Activation of protease by gibbrellin. Hakko Kyokaishi 19, 285–294 (1961) (cit. in) Chem. Abstr. 57, 11543 (1962)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mapelli, S., Ranieri, A.M. Total protein release from barley aleurone layers as a bioassay for gibberellins. Planta 142, 37–40 (1978). https://doi.org/10.1007/BF00385117
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00385117