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A cytokinin glucoside from the leaves of Phaseolus vulgaris L.

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Abstract

Phaseolus vulgaris plants decapitated above the primary leaves accumulate high cytokinin activity. The major cytokinin in these leaves was identified by Sephadex LH 20 chromatography, sensitivity to β-glucosidase and permanganate oxidation, and by combined gas chromatography-mass spectrometry as 6-(4-O-β-D-glucosyl-3-methylbutylamino) purine, (dihydrozeatin-O-β-D-glucoside). A possible reason for the persistance of this compound in the primary leaves is discussed.

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Abbreviations

BSA:

Bis-(trimethylsilyl)-acetamide

DHZ:

dihydrozeatin

DHZOG:

dihydrozeatin-O-β-D-glucoside

TMS:

trimethylsilyl

Z:

zeatin

References

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Author notes

  1. T. L. Wang

    Present address: Botany Department, University of Leicester, LE1 7RH, Leicester, UK

  2. A. G. Thompson

    Present address: The Polytechnic, Holly Bank Road, HD3 3BP, Huddersfield, UK

Authors and Affiliations

  1. Department of Botany and Microbiology, The University College of Wales, Penglais, SY23 3DA, Aberystwyth, UK

    T. L. Wang, A. G. Thompson & R. Horgan

Authors
  1. T. L. Wang
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  2. A. G. Thompson
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  3. R. Horgan
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Wang, T.L., Thompson, A.G. & Horgan, R. A cytokinin glucoside from the leaves of Phaseolus vulgaris L.. Planta 135, 285–288 (1977). https://doi.org/10.1007/BF00384901

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  • DOI: https://doi.org/10.1007/BF00384901

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