Skip to main content
SpringerLink
Log in

DNA and histone content of immature tracheary elements from cultured artichoke explants

  • Published:
Planta Aims and scope Submit manuscript

Abstract

Relative amounts of DNA and histone were determined by Feulgen microdensitometry and alkaline fast-green microdensitometry in differentiating tracheary elements in cultured explants of Jerusalem artichoke (Helianthus tuberosus L.) tubers. The absence of endopolyploidy in cultured artichoke tissue was confirmed, and the nuclei of tracheary elements were exclusively at the 2C level for both DNA and histones.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adamson, D.: Expansion and division in auxin-treated plant cells. Canad. J. Bot. 40, 719–744 (1962)

    Google Scholar 

  • Alfert, M., Geschwind, I.: A selective staining method for the basic proteins of cell nuclei. Proc. Nat. Acad. Sci. USA 29, 991–999 (1953)

    Google Scholar 

  • Antropova, E.N., Petrova, T.F., Bogdanov, Y.F.: Cytophotometric studies of histone and DNA in the mitotic cycle of tulip endosperm nuclei. Caryologia 23, 395–403 (1970)

    Google Scholar 

  • Avanzi, S., Maggini, F., Innocenti, A.M.: Amplification of ribosomal cistrons during the maturation of metaxylem in the root of Allium cepa. Protoplasma 76, 197–210 (1973)

    Google Scholar 

  • Innocenti, A.M.: Cyclic changes of histones/DNA ratio in differentiating nuclei of metaxylem cell line in Allium cepa root tip. Caryologia 28, 225–228 (1975)

    Google Scholar 

  • Innocenti, A.M., Avanzi, S.: Some cytological aspects of differentiation of metaxylem in the root of Allium cepa. Caryologia 24, 283–291 (1971)

    Google Scholar 

  • Lai, V., Srivastava, L.M.: Nuclear changes during differentiation of xylem vessel elements. Cytobiology 12, 220–243 (1976)

    Google Scholar 

  • List, A.: Some observations on DNA content and cell nuclear volume growth in the developing xylem cells of higher plants. Amer. J. Bot. 50, 320–329 (1963)

    Google Scholar 

  • Phillips, R., Torrey, J.G.: DNA levels in differentiating tracheary elements. Develop. Biol. 39, 322–325 (1974)

    Google Scholar 

  • Phillips, R., Dodds, J.H.: Rapid differentiation of tracheary elements in cultured explants of Jerusalem artichoke. Planta 135, 207–212 (1977)

    Google Scholar 

  • Roberts, L.W.: Cyto-differentiation in plants: xylogenesis as a model system. Cambridge: University Press 1976

    Google Scholar 

  • Turgeon, R.: Differentiation of wound vessel members without DNA synthesis, mitosis or cell division. Nature 257, 806–808 (1975)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany and Microbiology, University College London, Gower Street, WC1E 6BT, London, UK

    J. H. Dodds & R. Phillips

Authors
  1. J. H. Dodds
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Phillips
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dodds, J.H., Phillips, R. DNA and histone content of immature tracheary elements from cultured artichoke explants. Planta 135, 213–216 (1977). https://doi.org/10.1007/BF00384892

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00384892

Key words

Search

Navigation