Summary
Amylase isozyme polymorphisms were shown to exist in certain genetic stocks of Zea mays, using the technique, of starch gel electrophoresis. The earliest period of amylase activity was detected during the second day after germination of seeds. The third day after germination gave the best resolution of amylase activity in the form of distinct zones. Tissues examined from adult plants showed no amylase activity. Several hybrid patterns were of interest, but the most unique situation is the F1 hybrid AA9xAA2. One of the zones in this hybrid appears to be intermediate to the parental types, suggesting the formation of a hybrid enzyme. In the F1 hybrid AA7xP51 there are two zones of enzyme activity neither of which is present in the parental strains; this is also suggestive of hybrid enzymes with altered electrophoretic mobilities.
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References
Ashton, G. C., and A. W. Braden: Serum β-globulin polymorphism in mice. Aust. J. biol. Sci. 14, 243–253 (1961).
—: Serum amylase (thread protein) polymorphism in cattle. Genetics 51, 431–437 (1965).
Beckman, L., J. G. Scandalios, and J. L. Brewbaker: Genetics of leucine aminopeptidase isozymes in maize. Genetics 50, 899–904 (1964).
Berk, J. E.: Electrophoretic behaviour of rabbit serum amylase. Nature (Lond.) 200, 572–573 (1963).
Hunter, R. L., and C. L. Markert: Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels. Science 125, 1294–1295, (1957).
Kikkawa, H., and T. Ogita: Biochemical and genetical analysis of amylase in Drosophila melanogaster by means of agar-gel electrophoresis. Jap. J. Genet. 37, 394–395 (1962).
Markert, C. L., and F. Moller: Multiple forms of enzymes: Tissue, ontogenic and species specific patterns. Proc. nat. Acad. Sci. (Wash.) 45, 753–763 (1959).
McGeachin, R. L.: Immunochemical characterization of amylase isozymes. Ann. N. Y. Acad. Sci. 103, 1009–1013 (1963).
Scandalios, J. G.: Subunit dissociation and recombination of catalase isozymes. Proc. nat. Acad. Sci. (Wash.) 53, 1035–1040 (1965).
Sick, K., and J. T. Nielsen: Genetics of amylase isozymes in the mouse. Hereditas (Lund) 51, 291–296 (1964).
Smithies, O.: Zone electrophoresis in starch gels: Group variation in the serum proteins of normal human adults. Biochem. J. 61, 629–641 (1955).
Wroblewski, F. (ed.): Multiple molecular forms of enzymes. Ann. N. Y. Acad. Sci. 94, 655–1030 (1961).
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Supported by the U.S. Atomic Energy Commission under contract No. AT (11-1) 1338. Portion of this work was done in the Department of Genetics, University of Hawaii, under support from the U.S. National Institutes of Health
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Scandalios, J.G. Amylase isozyme polymorphism in maize. Planta 69, 244–248 (1966). https://doi.org/10.1007/BF00384876
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DOI: https://doi.org/10.1007/BF00384876