Summary
The specific activities of the enzymes cinnamic acid 4-hydroxylase and cytidine-5′-diphospho-choline: 1,2-diacylglycerol cholinephosphotransferase (E.C. 2.7.8.2) were determined in microsomal fractions from a) dark-grown and b) irradiated parsley (Petroselinum hortense Hoffm.) cell suspension cultures. Cinnamic acid 4-hydroxylase activity was increased 4-fold after light treatment, while cholinephosphotransferase activity was decreased by about one third of its original activity. It can be concluded that increased microsomal lecithin biosynthesis is not required for the light-induced increase of cinnamic acid 4-hydroxylase activity, although the latter enzyme has previously been found to require a lipid cofactor for activity.
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Abbreviations
- CDP:
-
Cytidine-5′-diphospho
References
Ben-Tal, Y., Varner, J. E.: An early response to gibberellic acid not requiring protein synthesis. Plant Physiol. 54, 813–816 (1974)
Büche, T., Sandermann, H.: Lipid dependence of plant microsomal cinnamic acid 4-hydroxylase. Arch. Biochem. Biophys. 158, 445–447 (1973)
Diesperger, H., Müller, C. R., Sandermann, H.: Rapid isolation of a plant microsomal fraction by Mg++-precipitation. FEBS Letters 43, 155–158 (1974)
Firn, R. D., Kende, H.: Some effects of applied gibberellic acid on the synthesis and degradation of lipids in isolated barley aleurone layers. Plant Physiol. 54, 911–915 (1974)
Hahlbrock, K., Ebel, J., Ortmann, R., Sutter, A., Wellmann, E., Grisebach, H.: Regulation of enzyme activities related to the biosynthesis of flavone glycosides in cell suspension cultures of parsley (Petroselinum hortense). Biochim. biophys. Acta (Amst.) 244, 7–15 (1971)
Hahlbrock, K., Schröder, J.: Light-induced changes of enzyme activities in parsley cell suspension cultures. Increased rate of synthesis of phenylalanine ammonia-lyase. Arch. Biochem. Biophys. 166, 47–53 (1975)
Johnson, K. D., Kende, H.: Hormonal control of lecithin synthesis in barley aleurone cells: regulation of the CDP-choline pathway by gibberellin. Proc. nat. Acad. Sci. (Wash.) 68, 2674–2677 (1971)
Koehler, D., Johnson, K. D., Varner, J. E., Kende, H.: Differential effects of mannitol on gibberellin-regulated phospholipid synthesis and enzyme activities of the CDP-choline pathway in barley aleurone cells. Planta (Berl.) 104, 267–271 (1972)
Lord, J. M., Kagawa, T., Moore, T. S., Beevers, H.: Endoplasmic reticulum as the site of lecithin formation in castor bean endosperm. J. Cell Biol. 57, 659–667 (1973)
Sandermann, H.: The reactivation of C55-isoprenoid alcohol phosphokinase apoprotein by lipids. Evidence for lipid hydration in lipoprotein function. Europ. J. Bichem. 43 415–422 (1974)
Wellmann, E., Schopfer, P.: Phytochrome-mediated de novo synthesis of phenylalanine ammonia-lyase in cell suspension cultures of parsley. Plant Physiol., in press, (1975)
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Scheel, D., Sandermann, H. On the mechanism of light induction of plant microsomal cinnamic acid 4-hydroxylase. Planta 124, 211–214 (1975). https://doi.org/10.1007/BF00384764
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DOI: https://doi.org/10.1007/BF00384764