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Widespread tissue distribution of rabbit calreticulin, a non-muscle functional analogue of calsequestrin

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Summary

Calreticulin was identified in a variety of rabbit tissues by Western blot analysis. Indirect immunofluorescence studies on cultured cells or frozen sections from the corresponding tissues revealed that the protein was distributed to the endoplasmic reticulum or sarcoplasmic reticulum. Calreticulin was found to be an abundant calcium-binding protein in non-muscle and smooth muscle cells and a constitutent calcium-binding protein in cardiac and skeletal muscle. From the immunoblot data, calreticulin may exist as an isoform in rabbit neural retina. The present study establishes the ubiquity of calreticulin in intracellular calcium binding.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, University of Toronto, M5S 1A8, Toronto, Ontario, Canada

    Suzanne Tharin, Ewa Dziak & Michal Opas

  2. Cardiovascular Disease Research Group, University of Alberta, T6G 2S2, Edmonton, Alberta, Canada

    Marek Michalak

  3. Department of Biochemistry, University of Alberta, T6G 2S2, Edmonton, Alberta, Canada

    Marek Michalak

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  1. Suzanne Tharin
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  2. Ewa Dziak
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  3. Marek Michalak
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  4. Michal Opas
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Tharin, S., Dziak, E., Michalak, M. et al. Widespread tissue distribution of rabbit calreticulin, a non-muscle functional analogue of calsequestrin. Cell Tissue Res 269, 29–37 (1992). https://doi.org/10.1007/BF00384723

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  • DOI: https://doi.org/10.1007/BF00384723

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