Summary
Trehalose-6-phosphate synthase is another example of an enzyme of carbohydrate metabolism, in Saccharomyces, which could be regulated by interconversion of forms. Deactivation was mediated both in vivo and in vitro by a cyclic AMP-dependent protein kinase. Reversibility of this process was obtained by a phosphatase treatment leading to an increase in activity. The phosphorylated, less active form of the enzyme proved to be more susceptible to activation by ATP.Mg. Mutants with well defined lesions in the cyclic AMP-dependent protein kinase system were used to corroborate our findings of a possible regulatory mechanism of trehalose-6-phosphate synthase activity by interconversion of forms.
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Abbreviations
- PMSF:
-
phenyl-methyl-sulfonyl fluoride
- G-6-P:
-
glucose-6-phosphate
- UDPG:
-
uridine-5′-diphosphoglucose
- PEP:
-
phosphoenol pyruvate
- NAD+ :
-
β-nicotinamine adenine dinueleotide
- ATP:
-
adenonise 5′-triphosphate
- cAMP:
-
adenosine 2′:3′-cyclic monophosphate
- MOPS:
-
3 (N-morpholino) propanesulfonic acid
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Panek, A.C., de Araujo, P.S., Moura Neto, V. et al. Regulation of the trehalose-6-phosphate synthase complex in Saccharomyces . Curr Genet 11, 459–465 (1987). https://doi.org/10.1007/BF00384607
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DOI: https://doi.org/10.1007/BF00384607