Abstract
During chloroplast development in the primary leaves of Phaseolus vulgaris, the extractable activity of adenosine 5′-phosphosulfate sulfotransferase increased ten-fold. When chloroplast development took place in air enriched with 3.5 μl H2S·l-1 there was a decrease in adenosine 5′-phosphosulfate sulfotransferase activity. Cyst(e)ine in concentrations up to 1 mM (in the external medium) did not affect the increase in adenosine 5′-phosphosulfate sulfotransferase activity in intact plants. In plants with excised roots, 0.75 mM cyst(e)ine inhibited this increase. In green primary leaves, H2S or cyst(e)ine treatment resulted in a decrease of extractable adenosine 5′-phosphosulfate sulfotransferase activity. In intact plants, this effect of cyst(e)ine was observed at a concentration of 1 mM, and in plants with excised roots, 0.25 mM had a comparable effect.
In developing plants, the extractable activities of O-acetyl-L-serine sulfhydrylase (EC 4.2.99.9) and ribulosebisphosphate carboxylase (EC 4.1.1.39.) were not affected by H2S or cyst(e)ine.
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Abbreviations
- APS:
-
adenosine 5′-phosphosulfate
- APSSTase:
-
adenosine 5′phosphosulfate sulfotransferase
- BSA:
-
bovine serum albumin
- DTE:
-
dithioerythritol
- EDTA:
-
ethylenediaminetetra-acetic acid
- OASSase:
-
O-acetyl-L-serine sulfhydrylase
- PAPS:
-
adenosine 3′-phosphate 5′-phosphosulfate
- POPOP:
-
1,4 Di 2-(5-phenyloxazolyl)-benzene
- PPO:
-
2,5-diphenyloxazol
- RubP:
-
ribulose-bisphosphate
- RubPCase:
-
ribulosebiphosphate carboxylase
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This is no. 8 in the series “Regulation of Sulfate Assimilation in Plants.” The term “cysteine” is used when it is clear that cystine is not involved; “cyst(e)ine” is used for an undefined mixture of cysteine and cystine. The concentrations are expressed in all cases relative to cysteine
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Wyss, HR., Brunold, C. Regulation of adenosine 5′-phosphosulfate sulfotransferase activity by H2S and cyst(e)ine in primary leaves of Phaseolus vulgaris L.. Planta 147, 37–42 (1979). https://doi.org/10.1007/BF00384588
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DOI: https://doi.org/10.1007/BF00384588