Abstract
The shoot apex of the terminal bud was studied in four successive physiological states: during dormancy, when dormancy breaks, during the third week after the break of dormancy, and during a later typical period of active growth. DNA content was measured in Feulgen-stained nuclei of the axial zone, of the lateral zone, and of the rib meristem. The mitotic index was established for each zone of the meristem. During the period of dormancy, all the nuclei of the meristem are in phase G 1 of the cell cycle and are blocked at the same point common to all nuclei. When dormancy breaks, this blockage is removed simultaneously and all nuclei in the shoot apex resume their cell cycles starting at the same point. The cycles remain synchronized for awhile. In the axial zone they remain synchronized until the third week after resumption of active growth.
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Baserga, R.: Multiplication and division in mammalian cells. New York: Dekker 1976
Booker, C.E., Dwivedi, R.S.: Ultrastructure of meristematic cells of dormant and relaxed buds in Tradescantia paludosa. Exp. Cell Res. 92, 255–261 (1973)
Brossard, D.: Le bourgeonnement épiphylle chez le Bryophyllum daigremontianum Berger. Ann. Sci. Nat. Bot. Biol. Vég. 12e série 14, 93–214 (1973)
Brunori, A., D'Amato, F.: The DNA content of nuclei embryo of dry seeds of Pinus pinea and Lactuca sativa. Caryologia 20, 153–161 (1967)
Corsi, G., Avanzi, S.: Cytochemical analyses on cellular differentiation in the root tip of Allium cepa. Caryologia 23, 381–394 (1970)
Cottignies, A.: Définition cytophysiologique de la dormance du méristème chez le Fraxinus excelsior L. Etude de l'activité mitotique et de la teneur en DNA nucléaire dans le point végétatif dormant. C.R. Acad. Sci. D 218, 2763–2766 (1974a)
Cottignies, A.: Les critères nucléaires d'une dormance vraie et totale dans le point végétatif du Fraxinus excelsior L. Planta 120, 171–179 (1974b)
Dagnélie, P.: Théories et méthodes statistiques. Gembloux: Presses agronomiques 1970
Dwivedi, R.S., Naylor, J.M.: Influence of apical dominance on the nuclear proteines in cells of the lateral bud meristem in Tradescantia paludosa. Can. J. Bot. 46, 289–298 (1968)
Gabe, M.: Techniques histologiques. Masson, Paris (1968)
Gelfant, S.: A new concept of tissue and tumor cell proliferation. Cancer Res. 37, 3845–3862 (1977)
Lajtha, L.G.: Differential sensitivity of the cell life cycle. J. Cell. Comp. Physiol. 62, suppl. 1, 141–156 (1963)
Martin, R.G., Stein, S.: Resting states in normal and simian virus 40 transformed chinese hamster lung cells. Proc. Natl. Acad. Sci. USA 73, 1655–1659 (1976)
Michaux, N.: Structure et fonctionnement du méristème apical des Ptéridophytes. Paris: Thèse, Masson et Cie 1971
Michaux, N.: Etude cytophotométrique du méristème caulinaire de l'Isoetes setacea Lam. au cours de son cycle annuel. C.R. Acad. Sci. D 274, 3453–3456 (1972)
Muroyama, T., Fujita, H., Takajana, Mishima, A., Maruyama, Y.: Division cycle of Bacillus subtilis cells as studied by method of synchronous culture. Jpn. J. Genet. 44, 399 (1969)
Naylor, J.M.: Control of nuclear process by auxin in axillary buds of Tradescantia paludosa. Can. J. Bot. 36, 221–232 (1958)
Nougarède, A.: Infrastructure des axillaires cotylédonnaires du Pisum sativum L. (var. nain hâtif d'Annonay) durant le blocage en phase G1 (état inhibé) et après la reprise d'activité. C.R. Acad. Sci. D 284, 25–28 (1977)
Nougarède, A., Rondet, P.: Index mitotiques en teneurs en DNA nucléaire du méristème axillaire de la feuille rang 6 et du point végétatif terminal, chez le Pisum sativum L. C.R. Acad. Sci. D 280, 709–712 (1975a)
Nougarède, A., Rondet, P.: Inhibition in the Pisum sativum axillary buds and release bu ablation of the main stem, pp. 305. In: Abstr. XII International Botanical Congress, Leningrad 1975b
Nougarède, A., Tepfer, S., Tepfer, M.: Les bourgeons au cours de l'état pérenaant chez le Phytolacca decandra L. C.R. Acad. Sci. D 276, 957–960 (1973a)
Nougarède, A., Tepfer, S., Tepfer, M.: Seasonal changes in the shoot apex of Phytolacca L. Am. J. Bot. 60, 4–11 (1973b)
Owens, J.N., Molder, M.: A study of DNA and mitotic activity in the vegetative apex of Douglas fir during the annual growth cycle. Can. J. Bot. 51, 1395–1409 (1973)
Pardee, A.B.: Restriction point for control of normal animal cell proliferation. Proc. Natl. Acad. Sci. USA 71, 1296–1290 (1974)
Patau, K.: Absorption microphotometry of irregular-shaped objects. Chromosoma 5, 341–362 (1952)
Radley, J.M., Hodgson, G.S., Koschel, K.W.: Properties of Go cells: variations in the proliferative response following isoprenaline. Cell Tissue Kinet. 9, 371–377 (1976)
Rembur, J.: Etude autoradiographique et cytophotométrique de la synthèse du DNA au cours des premières heures de la germination chez le Xanthium. C.R. Acad. Sci. D 271, 908–911 (1970)
Saint-Côme, R.: Durée du cycle mitotique dans le point végétatif du Coleus Blumei Benth. C.R. Acad. Sci. D 268, 508–511 (1969)
Sallé, G.: Etude cytologique, cytochimique et histoautoradiographique du Viscum album L. Paris: Thèse, Université Pierre et Marie Curie 1977
Schwartz, D.: Méthodes statistiques à l'usage des médecins et des biologistes, 3ème éd. Paris: Flammarion 1969
Short, K.C., Brown, E.G., Street, H.E.: Studies on the growth in culture of plant cells. VI. J. Exp. Bot. 20, 579–591 (1969)
Thomas, M.J.: Etude cytophotométrique du DNA nucléaire des différents territoires de l'embryon du Pinus silvestris L. au cours de son développement. C.R. Acad. Sci. D 280, 1087–1090 (1975)
Toupiol, D.: Mise en place de l'inhibition du bourgeon cotylédonnaire chez le Pisum sativum L. C.R. Acad. Sci. D 282, 281–284 (1976)
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Cottignies, A. The blockage in the G 1 phase of the cell cycle in the dormant shoot apex of ash. Planta 147, 15–19 (1979). https://doi.org/10.1007/BF00384584
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DOI: https://doi.org/10.1007/BF00384584