Summary
Protoplast fusion was carried out by using a routine technique with various auxotrophic strains of Saccharomyces diastaticus and Schizosaccharomyces pombe, and abortive fusion products were observed as small colonies which appeared more frequently than large prototrophic colonies. Sixty abortive fusion products retained one or more auxotrophic characters derived from S. diastaticus, one of the strains used in the protoplast fusion. Several hybrids were obtained between the abortive products and S. cerevisiae, and the segregants of these hybrids showed many aberrant tetrads with regard to some genetic markers. These segregation patterns would be likely to result if the segregating characters were in the trisomic condition +/+/-. The results indicate that (1) the abortive fusion products are an alien monosome additional (AMA) haploid containing the genome of S. diastaticus and only one chromosome of S. pombe. (2) The additional chromosome of S. pombe, which is integrated with the genome of Saccharomyces, can be stably transmitted to the progeny.
References
DeMoss JA (1965) Biochemical diversity in the tryptophan pathway. Biochem Biophys Res Commun 18:850–857
Emerson S (1956) Notes on the identification of different causes of aberrant tetrad ratios in Saccharomyces. CR Trav Lab Carlsberg Sér Physiol 26:71–86
Foury F, Goffeau A (1973) Combination of 2-deoxyglucose and snail-gut enzyme treatments for preparing sphaeroplasts of Schizosaccharomyces pombe, J Gen Microbiol 75:227–229
Gunge N, Tamaru A (1978) Genetic analysis of products of protoplast fusion in Saccharomyces cerevisiae. Jpn J Genet 53:41–49
Jones EE, Broquist HP (1965) Saccharopine, an intermediate of the aminoadipic acid pathway of lysine biosynthesis. J Biol Chem 240:2531–2536
Kohli J, Hottinger H, Munz P, Struss A, Thuriaux P (1977) Genetic mapping in Schizosaccharomyces pombe by mitotic and meiotic analysis and induced haploidization. Genetics 87:471–489
Kucsera J, Ferenczy L (1979) Interspecific protoplast fusion between Candida albicans and Candida tropicalis. Abstr 5th Int Protoplast Symp, Hungary, p 52
Mortimer RK, Hawthorne DC (1973) Genetic mapping in Sacchromyces IV. Mapping of temperature-sensitive genes and use of disomic strains in localizing genes. Genetics 74:33–54
Novák EK, Pólay A, Becalel-Vajda M, Sztankov L (1979) Immuno-cytochemical demonstration of interspecific protoplast fusion between Candida albicans and Saccharomyces cerevisiae. Abstr 5th Int Protoplast Symp, Hungary, p 90
Provost A, Bourguignon C, Fournier P, Ribet AM, Heslot H (1978) Intergeneric hybridization in yeasts through protoplast fusion. FEMS Microbiol Lett 3:309–312
Satyanarayana T, Umbarger HE, Lindegren G (1968) Biosynthesis of branched-chain amino acids in yeast: correlation of biochemical blocks and genetic lesions in leucine auxotrophs. J Bacteriol 96:2012–2017
Davchenko GV, Kapul'tsevich YuG (1979) Hybridization of yeasts from genes Hansenula by protoplast fusion. Abstr 5th Int Protoplast Symp, Hungary, p 55
Schweingruber ME, Dietrich R (1973) Gene-enzyme relationships in the tryptophan pathway of Schizosaccharomyces pombe. Experientia 29:1152–1154
Svoboda, A. (1979) Intergeneric fusion of protoplasts: Schizosaccharomyces pombe+Saccharomyces cerevisiae. Abstr 5th Int Protoplast Symp, Hungary, p 89
Takahashi T, Saito H, Ikeda Y (1958) Heterothallic behavior of a homothallic strain in Saccharomyces yeast. Genetics 43:249–260
Tamaki H (1978) Genetic studies of ability to ferment starch in Saccharomyces: gene polymorphism. Mol Gen Genet 164:205–209
Tamaki H (1980) Interspecific protoplast fusion between Saccharomyces diastaticus and S. rouxii. Jpn J Genet 55:492 Abstr
Vallin C, Kucsera J, Klinner U, Ferenczy L (1979) Intergeneric protoplast fusion between Candida tropicalis and Pichia guilliermondii. Abstr 5th Int Protoplast Symp, Hungary, p 59
Whittaker PA, Leach SM (1978) Interspecific hybrid production between the yeasts Kluyveromyces lactis and Kluyveromyces fragilis by protoplast fusion. FEMS Microbiol Lett 4:31–34
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Tamaki, H. Genetic properties of abortive products resulting from the protoplast fusion in yeasts. Molec. Gen. Genet. 187, 177–179 (1982). https://doi.org/10.1007/BF00384404
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00384404