Summary
Acid phosphatase in S. cerevisiae exists as an enzymatically active, cell wall associated form and as an enzymatically inactive, probably membrane-bound form (Schweingruber and Schweingruber, in press). Orthophosphate dependent and independent regulation determines the level of acid phosphatase activity. To deduce the regulation mechanisms we purified and quantified active and inactive acid phosphatase from cells grown under different physiological conditions and displaying variable levels of enzyme activity. Orthophosphate dependent regulation does not include significant changes in the amount of total (active and inactive) acid phosphatase protein synthesized. Under the experimental conditions chosen increased activity is achieved by preferential synthesis of the active form and by increasing the specific activity of the active enzyme. Orthophosphate independent regulation seems to occur by similar mechanisms.
Similar content being viewed by others
References
Ballou C (1976) Structure and biosynthesis of the mannan component of the yeast cell envelope. Adv Microb Physiol 14:93–158
Ballou L, Cohen RE, Ballou CE (1980) Saccharomyces cerevisiae mutants that make mannoproteins with a truncated carbohydrate outer chain. J Biol Chem 255:5986–5991
Boer P, van Rijn HJ, Reinking A, Steyn-Parvé EP (1975) Biosynthesis of acid phosphatase of baker's yeast. Characterization of a protoplast-bound fraction containing precursors of the exo-enzyme. Biochim Biophys Acta 377:331–342
Boer P, Steyn-Parvé EP (1966) Isolation and purification of an acid phosphatase from baker's yeast (Saccharomyces cerevisiae). Biochim Biophys Acta 128:400–402
Bostian KA, Lemire JM, Cannon LE, Halvorson HO (1980) In vitro synthesis of represible yeast acid phosphatase: Identification of multiple mRNAs and products. Proc Natl Acad Sci USA 77:4504–4508
Dibenedetto G, Cozzani I (1975) Nonspecific acid phosphatase from Schizosaccharomyces pombe. Biochemistry 14:2847–2852
Linnemans WAM, Boer P, Elbers PF (1977). Localization of acid phosphatase in Saccharomyces cerevisiae: a clue to cell wall formation. J Bacteriol 131:638–644
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Rainina EJ, Zubatov AS, Buchalow IB, Luzikov VH (1979) A cytochemical study of the localization of acid phosphatase in Saccharomyces cerevisiae at different growth phases. Histochem J 11:299–310
van Rijn NJM, Linnemans WAM, Boer P (1975) Localization of acid phosphatase in protoplasts from Saccharomyces cerevisiae. J Bacteriol 123:1144–1149
Roe JH (1955) The determination of sugar in blood and spinal fluid with anthrone reagent. J Biol Chem 212:335–343
Rubin GM (1973) The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J Biol Chem 248:3860–3875
Schurr A, Yagil E (1971) Regulation and characterization of acid and alkaline phosphatase in yeast. J Gen Microbiol 65:291–303
Schweingruber ME, Schweingruber AM (1979) Posttranslational regulation of repressible acid phosphatase in yeast. Mol Gen Genet 173:349–351
Schweingruber ME, Schweingruber AM (1981) Modulation of a cell surface glycoprotein in yeast. Acid phosphatase. Differentiation 19:68–70
Schweingruber ME, Schweingruber AM (1982) Purification and identification of inactive forms of repressible and constitutive acid phosphatase in yeast. Biochim Biophys Acta, in press
Schweingruber AM, Schweingruber ME, Schüpbach ME (1982) Isolation and characterization of acid phosphatase mutants in Schizozaccharomyces pombe. Curr Genetics 5:109–117
Toh-e A, Inouye S, Oshima Y (1981) Structure and function of the PHO82-pho4 locus controlling the synthesis of repressible acid phosphatase of Saccharomyces cerevisiae. J Bacteriol 145:221–232
Toh-e A, Kabayashi S, Oshima Y (1978) Disturbance of the machinery for the gene expression by acidic pH in the repressible acid phosphatase system of Saccharomyces cerevisiae. Mol Gen Genet 162:139–149
Toh-e A, Kakimoto S, Oshima Y (1975) Genes coding for the structure of the acid phosphatases in Sacharomyces cerevisiae. Mol Gen Genet 143:65–70
Toh-e A, Oshima Y (1974) Characterization of a dominant, constitutive mutation, PHOO, for the repressible acid phosphatase synthesis in Saccharomyces cerevisiae. J Bacteriol 120:608–617
Toh-e A, Ueda Y, Oshima Y (1973) Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae. J Bacteriol 113:727–738
Ueda Y, Oshima Y (1975) A constitutive mutation, phoT, of repressible acid phosphatase synthesis with inability to transport inorganic phosphate in Saccharomyces cerevisiae. Mol Gen Genet 136:255–259
Ueda Y, Toh-e A, Oshima Y (1975) Isolation and characterization of recessive mutations for repressible acid phosphatase synthesis in Saccharomyces cerevisiae. J Bacteriol 122:911–922
Welten-Verstegen GW (1981) The role of the plasmamembrane in glycosylation of proteins in yeast. PH-D Thesis, Utrecht HL
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Schweingruber, AM., Schweingruber, M.E. Differential regulation of the active and inactive forms of Saccharomyces cerevisiae acid phosphatase. Molec. Gen. Genet. 187, 107–111 (1982). https://doi.org/10.1007/BF00384391
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00384391