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Sexual agglutination factor from Chlamydomonas eugametos

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Abstract

Chlamydomonas eugametos gametes can sexually agglutinate via their flagellar surfaces whereas vegetative cells cannot. Therefore, flagellar glycoproteins, present in gamete cells but absent from vegetative cells, were investigated as prospective mt -agglutination factors. They were identified as periodic acid Schiff (PAS) stained bands separated in sodium dodecyl sulphate-polyacrylamide electrophoresis gels. Gamete-specific bands were determined by comparison with equivalent gels of vegetative flagella and by immunological techniques using antisera raised against isolated mt - gamete flagella. Four high molecular weight flagellar glycoproteins proved to be gamete specific (PAS-1.2, PAS-1.3, PAS-3 and PAS-4). They were extracted from flagella by 3 M guanidine thiocyanate, separated in a column of Sepharose 2B, and tested for in vitro agglutination activity on mt + gametes. A single peak of activity was found to be correlated with the presence of the PAS-1.2 band. It is shown that mt - agglutination activity is related to the concentration of this glycoprotein in flagellar membranes.

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Abbreviations

SDS:

sodium dodecyl sulphate

PAS:

periodic acid Schiff

GTC:

guanidine thiocyanate

mt -/+ :

mating type plus or minus

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Authors and Affiliations

  1. Plant Physiology Department, University of Amsterdam, Kruislaan 318, NL-1098 SM, Amsterdam, The Netherlands

    Alan Musgrave, Ed van Eijk, Ruud te Welscher, Rob Broekman & Peter Lens

Authors
  1. Alan Musgrave
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  2. Ed van Eijk
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  3. Ruud te Welscher
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  4. Rob Broekman
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  5. Peter Lens
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Musgrave, A., van Eijk, E., te Welscher, R. et al. Sexual agglutination factor from Chlamydomonas eugametos . Planta 153, 362–369 (1981). https://doi.org/10.1007/BF00384255

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  • DOI: https://doi.org/10.1007/BF00384255

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