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Turgor pressure and water transport properties of suspension-cultured cells of Chenopodium rubrum L.

Planta volume 151, pages 95–102 (1981)Cite this article

Abstract

The turgor pressure and water relation parameters were determined in single photoautotrophically grown suspension cells and in individual cells of intact leaves of Chenopodium rubrum using the miniaturized pressure probe. The stationary turgor pressure in suspension-cultured cells was in the range of betwen 3 and 5 bar. From the turgor pressure relaxation process, induced either hydrostatically (by means of the pressure probe) or osmotically, the halftime of water exchange was estimated to be 20±10 s. No polarity was observed for both ex- and endosmotic water flow. The volumetric elastic modulus, ε, determined from measurements of turgor pressure changes, and the corresponding changes in the fractional cell volume was determined to be in the range of between 20 and 50 bar. ε increases with increasing turgor pressure as observed for other higher plant and algal cells. The hydraulic conductivity, Lp, is calculated to be about 0,5–2·10−6 cm s−1 bar−1. Similar results were obtained for individual leaf cells of Ch. rubrum. Suspension cells immobilized in a cross-linked matrix of alginate (6 to 8% w/w) revealed the same values for the half-time of water exchange and for the hydraulic conductivity, Lp, provided that the turgor pressure relaxation process was generated hydrostatically by means of the pressure probe. Thus, it can be concluded that the unstirred layer from the immobilized matrix has no effect on the calculation of Lp from the turgor pressure relaxation process, using the water transport equation derived for a single cell surrounded by a large external volume. By analogy, this also holds true for Lp-values derived from turgor pressure changes generated by the pressure probe in a single cell within the leaf tissue. The fair similarity between the Lp-values measured in mesophyll cells in situ and mesophyll-like suspension cells suggests that the water transport relations of a cell within a leaf are not fundamentally different from those measured in a single cell.

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Author information

Affiliations

  1. Kernforschungsanlage Jülich, Arbeitsgruppe Membranforschung am Institut für Medizin, Postfach 1913, D-5170, Jülich 1, Germany

    K.-H. Büchner & U. Zimmermann

  2. Institut für Biologie I der Universität, Auf der Morgenstelle 1, D-7400, Tübingen, Germany

    F.-W. Bentrup

Authors
  1. K.-H. Büchner
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  2. U. Zimmermann
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  3. F.-W. Bentrup
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Büchner, KH., Zimmermann, U. & Bentrup, FW. Turgor pressure and water transport properties of suspension-cultured cells of Chenopodium rubrum L.. Planta 151, 95–102 (1981). https://doi.org/10.1007/BF00384243

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  • DOI: https://doi.org/10.1007/BF00384243

Key words

  • Cell suspension culture
  • Chenopodium
  • Elasticity
  • Immobilization
  • Pressure probe
  • Water relations