Abstract
Incorporation of [14C]uridine into ribosomal RNA and ribosomal RNA precursor fractions was studied in isolated wheat embryos and in embryos remaining within the intact grains during germination. In isolated embryos, radioactive 25S and 18S as well as 31S RNA species appeared already within the first 3 h of germination. In non-isolated embryos, radioactivity could be detected in the mature ribosomal and pre-ribosomal RNA fractions only after 6 h and 12 h germination, respectively. It is concluded that transcription of ribosomal sequences is activated almost immediately in isolated wheat embryos but remains repressed for several hours when the embryo germinates under natural physiological conditions.
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Abbreviations
- HnRNA:
-
heterogeneous nuclear RNA
- mRNA:
-
messenger RNA
- pre-rRNA:
-
precursor of ribosomal RNA
- rRNA:
-
ribosomal RNA
- SDS:
-
sodium dodecyl sulphate
References
Bhat, S.P., Padayatty, J.D.: Transcriptional events during early phase of germination of rice embryos. Nature 256, 227–228 (1975)
Chen, D., Schultz, G., Katchalski, E.: Early ribosomal RNA transcription and appearance of cytoplasmic ribosomes during germination of the wheat embryo. Nature New Biol. 231, 69–72 (1971)
Cherry, J.H., Chroboczek, H.: Factors affecting nucleic acid extractability in plants. Phytochemistry 5, 411–422 (1966)
Dobrzańska, M., Buchowicz, J.: High molecular weight UMP-rich RNA of germinating wheat embryo. Biochim. Biophys. Acta 432, 73–79 (1976)
Dobrańska, M., Tomaszewski, M., Grzelczak, Z., Rejman, E., Buchowicz, J.: Cascade activation of genome transcription in wheat. Nature 244, 507–509 (1973)
Johnston, F.B., Stern, H.: Mass isolation of viable wheat embryos. Nature 179, 160–161 (1957)
Loeniug, U.E.: The fractionation of high molecular weight ribonucleic acid by polyacrylamide gel electrophoresis. Biochem. J. 102, 251–257 (1967)
Rejman, E., Buchowicz, J.: The sequence of initiation of DNA, RNA and protein synthesis in the wheat grains during germination. Phytochemistry, 10, 2951–2957 (1971)
Rejman, E., Buchowicz, J.: RNA synthesis during the germination of wheat seed. Phytochemistry 12, 271–276 (1973)
Sen, S., Payne, P.I., Osborne, D.J.: Early ribonucleic acid synthesis during the germination of rye (Secale cereale) embryos and the relationship to early protein synthesis. Biochem. J. 148, 381–387 (1975)
Silverstein, E.: Subribosomal RNP particles of developing wheat embryo. Biochemistry 12, 951–958 (1973)
Spiegel, S., Obendorf, R.L., Marcus, A.: Transcription of ribosomal and messenger RNAs in early wheat embryo germination. Plant Physiol. 56, 502–507 (1975)
Van De Walle, C.: MAK column chromatography of the first RNA synthesized during germination of Zea mays embryos. FEBS Lett. 16, 219–221 (1971)
Van De Walle, C., Bernier, G., Deltour, R., Bronchart, R.: Sequence of reactivation of ribonucleic acid synthesis during early germination of the maize embryo. Plant Physiol. 57, 632–639 (1976)
Van Onckelen, H.A., Verbeek, R., Khan, A.A.: Relationship of ribonucleic acid metabolism in embryo and aleurone to α-amylase synthesis in barley. Plant Physiol. 53, 562–568 (1974)
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Grzelczak, Z., Buchowicz, J. A comparison of the activation of ribosomal RNA synthesis during germination of isolated and non-isolated embryos of Triticum aestivum L.. Planta 134, 263–265 (1977). https://doi.org/10.1007/BF00384191
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DOI: https://doi.org/10.1007/BF00384191