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DNA sequence of an amber replication mutant indicates that a 29 kd protein is the product of the F plasmid replication gene

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Summary

DNA sequencing shows that the mutational alteration resulting from an amber-suppressible replication-defective mutation of F plasmid is a single base pair change from C:G to T:A which yields an amber codon in the coding frame for a 29 kd polypeptide located in the minimal replication region. We thus identified the gene indispensable for F DNA replication as the coding frame which encodes a 29 kb polypeptide. We will designate this gene repA.

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Abbreviations

kb:

kilobase pairs

bp:

base pair

kd:

kilodaltons

References

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Author notes

  1. Satoko Maki

    Present address: Department of Biochemistry, Stanford University, School of Medicine, 94305, Stanford, California, USA

Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kyushu University, Maedashi, Higashi-ku, 812, Fukuoka, Japan

    Satoko Maki, Takeyoshi Miki & Tadao Horiuchi

Authors
  1. Satoko Maki
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  2. Takeyoshi Miki
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  3. Tadao Horiuchi
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Additional information

Communicated by T. Yura

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Maki, S., Miki, T. & Horiuchi, T. DNA sequence of an amber replication mutant indicates that a 29 kd protein is the product of the F plasmid replication gene. Molec. Gen. Genet. 194, 337–339 (1984). https://doi.org/10.1007/BF00383537

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  • DOI: https://doi.org/10.1007/BF00383537

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