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Studies of ribosomes of yeast species: Susceptibility to inhibitors of protein synthesis in vivo and in vitro

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Summary

The susceptibility of cells from various species of Saccharomyces and Kluyveromyces was tested against different types of protein synthesis inhibitors. Minimal inhibitory concentrations were determined for each yeast species and the sensitivity of their ribosomes in cell-free extracts was tested. Two aminoglycosides, paromomycin and hygromycin B were assayed for capacity to stimulate translation errors with ribosomes of yeast species showing different minimal inhibitory concentrations in vivo. In many cases a correlation exists between natural and in vitro resistance suggesting that some natural antibiotic resistances are ribosomal.

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Authors and Affiliations

  1. Laboratoire de Génétique de l'Université de Paris-Sud, Centre d'Orsay (Laboratoire associé au CNRS n°86), Bâtiment 400, F-91405, Orsay Cedex, Frahce

    Arlette Adoutte-Panvier

  2. Biogen S.A., 46 Route des Acacias, CH-1227, Carouge/Geneva, Switzerland

    Julian E. Davies

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  1. Arlette Adoutte-Panvier
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  2. Julian E. Davies
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Communicated by A. Böck

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Adoutte-Panvier, A., Davies, J.E. Studies of ribosomes of yeast species: Susceptibility to inhibitors of protein synthesis in vivo and in vitro. Molec. Gen. Genet. 194, 310–317 (1984). https://doi.org/10.1007/BF00383533

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  • DOI: https://doi.org/10.1007/BF00383533

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