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Control of Herpes simplex virus thymidine kinase gene expression in Saccharomyces cerevisiae by a yeast promoter sequence

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Summary

This study presents the first evidence that the 5′ promoter region of the Saccharomyces cerevisiae glyceraldehyde-3-phosphate dehydrogenase gene (G-3-PD) promoter will permit expression of an adjacent foreign gene. The S. cerevisiae G-3-PD promoter was linked to the herpes simplex virus — thymidine kinase (HSV-TK) gene in a shuttle plasmid capable of autonomous replication in both yeast and Escherichia coli. Since the HSV-TK gene promoter is not functional in yeast, yeast cells containing these plasmids will express the HSV-TK gene and synthesize thymidine kinase only if the yeast promoter fragment is fused to the HSV-TK gene in the proper orientation. The 5′ flanking sequences necessary for the expression of heterologous eukaryotic genes in S. cerevisiae are discussed.

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  1. Xin-Liang Zhu

    Present address: Shanghai Institute of Cell Biology, 320 Yoyang Road, Shanghai, China

Authors and Affiliations

  1. Department of Cell Biology, Roche Institute of Molecular Biology, Nutley, New Jersey, USA

    Xin-Liang Zhu, Cheryl Ward & Arthur Weissbach

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  1. Xin-Liang Zhu
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  2. Cheryl Ward
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Communicated by G. Fink

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Zhu, XL., Ward, C. & Weissbach, A. Control of Herpes simplex virus thymidine kinase gene expression in Saccharomyces cerevisiae by a yeast promoter sequence. Molec. Gen. Genet. 194, 31–41 (1984). https://doi.org/10.1007/BF00383493

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  • DOI: https://doi.org/10.1007/BF00383493

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