Summary
Complementation experiments with cloned DNA fragments encoding either the KS71A, the KS71B or the KS71C fimbriae of the pyelonephritogenic Escherichia coli strain KS71 were used to localise the P-fimbrillin genes and to demonstrate regulatory interactions between the cloned genes. The structural genes of the KS71A and KS71B fimbriae were located within a common 1.1 kilobase pair ClaI-SmaI fragment, and it was shown that the gene clusters for these fimbriae could complement each other in trans. The gene cluster encoding the KS71C fimbriae did not complement for the other KS71 fimbriae. A DNA fragment, located near the KS71A fimbrillin gene, was found to enhance the production of the KS71B fimbriae in trans.
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Altenbuchner J, Schmid K, Schmitt R (1983) Tn1721-encoded tetracycline resistance: Mapping of structural and regulatory genes mediating resistance. J Bacteriol 153:116–123
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening of recombinant plasmid DNA. Nucleic Acids Res 7:1513–1517
Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heynecker HL, Boyer HW, Crosa JH, Falkow S (1977) Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
Chang AC, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol 134:1141–1156
Evans DG, Evans DJ, Jr (1978) New surface-associated colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups 06 and 08. Infect Immun 21:638–647
Källenius G, Svenson SB, Möllby R, Cedergren B, Hultberg H, Winberg H (1981) Structure of the carbohydrated part on human uroepithelial cells for pyelonephritogenic Escherichia coli. Lancet II:604–606
Klemm P, Ørskov I, Ørskov F (1982) F7 and type-1 like fimbriae from three Escherichia coli strains isolated from urinary tract infection. Infect Immun 36:462–468
Korhonen TK, Väisänen V, Saxen H, Hultberg H, Svenson SB (1982) P-antigen-recognizing fimbriae from human uropathogenic Escherchia coli strains. Infect Immun 37:286–291
Mandel M, Higa A (1970) Calcium dependent bacteriophage DNA infection. J Mol Biol 53:154–162
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, New York
Mooi FR, Harms N, Bakker D, De Graaf FK (1981) Organization and expression of genes involved in the production of the K88ab antigen. Infect Immun 32:1155–1163
Normark S, Lark D, Hull R, Norgren M, Båga M, O'Hanley P, Schoolnik G, Falkon S (1983) Genetics of a digalactoside binding adhesin from a uropathogenic Escherichia coli strain. Infect Immun 41:942–948
Nowicki B, Rhen M, Väisänen-Rhen V, Pere A, Korhonen TK (1984) Immunofluorescence study of fimbrial phase variation in Escherichia coli KS71. J Bacteriol 160:691–695
Rhen M (1985) Characterization of DNA fragments encoding fimbriae of the uropathogenic Escherichia coli strain KS71. J Gen Microbiol 131:571–580
Rhen M, Klemm P, Wahlström E, Svenson SB, Källenius G, Korhonen TK (1983a) P-fimbriae of Escherichia coli: Immunoand protein-chemical characterization of fimbriae from two pyelonephritogenic strains. FEMS Microbiol Lett 18:233–238
Rhen M, Knowles J, Penttilä ME, Sarvas M, Korhonen TK (1983b) P-fimbriae of Escherichia coli. Molecular cloning of DNA-fragments containing the structural genes. FEMS Microbiol Lett 19:119–123
Rhen M, Mäkelä PH, Korhonen TK (1983c) P-fimbriae of Escherichia coli are subject to phase variation. FEMS Microbiol Lett 19:267–271
Svanborg Edén C, Hansson HA (1978) Escherichia coli pili as mediators of attachment to urinary tract epithelial cells. Infect Immun 21:229–237
Svanborg Edén C, Hanson LÅ, Jodal U, Linberg U, Sohl-Åkerlund A (1976) Variable adhesion to normal urinary tract epithelial cells of Escherichia coli strains associated with various forms of urinary tract infection. Lancet II:490–492
Väisänen V, Elo J, Tallgren LC, Siitonen A, Mäkelä PH, Svanborg Eden C, Källenius G, Svenson SB, Hultberg H, Korhonen TK (1981) Mannose-resistant hemagglutination and P antigen recognition are characteristics of Escherichia coli causing primary pyelonephritis. Lancet II:1366–1369
Van Die I, Van Megen I, Hoekstra W, Bergmans H (1984) Molecular organisation of the genes involved in the production of F72 fimbriae, causing mannose resistant haemagglutination, of a uropathogenic Escherichia coli 06:K2:H1:F7 strain. Mol Gen Genet 194:528–533
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Communicated by P.T. Emmerson
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Rhen, M., Väisänen-Rhen, V., Pere, A. et al. Complementation and regulatory interaction between two cloned fimbrial gene clusters of Escherichia coli strain KS71. Molec. Gen. Genet. 200, 60–64 (1985). https://doi.org/10.1007/BF00383312
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DOI: https://doi.org/10.1007/BF00383312