Skip to main content
Log in

Studies on the excystment of trematode metacercariae in vitro

  • Published:
Zeitschrift für Parasitenkunde Aims and scope Submit manuscript

Summary

Excystment of metacercariae of Parorchis acanthus, Posthodiplostomoides leonensis, Posthodiplostomum sp. and Clinostomum tilapiae was found to be an active process initiated by the metacercariae when the correct physico-chemical conditions exist in the environment; but in the excystment of Posthodiplostomum nanum the metacercaria seems not to take part in the process.

Temperature, osmotic pressure, pH and eH are factors which influence all the five species studied. Fresh ox bile was found not to be an absolute necessity for the excystment of the five species studied but its presence in the medium increased the rate of excystment of three species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Asanji, M. F., Williams, M. O.: The structure and histochemistry of some trematode metacercarial cysts. J. Helminth. 47, 353–368 (1973)

    Google Scholar 

  • Asanji, M. F., Williams, M. O.: Studies on the Excystment of trematode metacercariae in vivo. J. Helminth. 48, 85–91 (1974)

    PubMed  Google Scholar 

  • Cameron, G.: Tissue culture technique. New York: Academic Press Inc. 1950

    Google Scholar 

  • Dawes, B.: On the early stages of Fasciola hepatica penetrating into the liver on an experimental host, the mouse, a histological picture. J. Helminth. R. T. Leiper Suppl., p. 41–52 (1961)

  • Dixon, K. E.: Excystment of metacercariae of Fasciola hepatica L. in vitro. Nature (Lond.) 202, 1240–1241 (1964)

    Google Scholar 

  • Dixon, K. E.: The physiology of excystment of metacercariae of Fasciola hepatica L. Parasitology 56, 431–456 (1966)

    Google Scholar 

  • Erasmus, D. A.: The biology of trematodes. London: Edward Arnold 1972

    Google Scholar 

  • Howell, M. J.: Excystment and in vitro cultivation of Echinoparyphium serratum. Parasitology 58, 583–597 (1968)

    Google Scholar 

  • Hsu, H. F., Wang, L. S.: Studies on certain problems of Clonorchis sinensis. IV. Notes on the resistance of cysts in fish flesh, the migration route and the morphology of the young worms in the final host. Chin. med. J. Suppl. 2, 385–400 (1938)

    Google Scholar 

  • Hunter, W. C., Chait, D. C.: Notes on excystment and culture in vitro of the microphallid Trematode Gynaecotyle adunca (Linton, 1905). J. Parasit. 38, 87 (1952)

    Google Scholar 

  • Kobayashi, A., et al.: Studies on excystment of the metacercariae of Metagonismus yokogawai. Acta Sch. med. Gifu 7, 822–828 (1959) (Abstract in: Helminth. Abstr. 29, 221)

    Google Scholar 

  • Macy, R. W.: Studies on the taxonomy, morphology and biology of Prosthogonimus macrorchis Macy, a common avain fluke of domestic fowls in North America. Univ. Minn. technol. Bul. 98, 1–11 (1934)

    Google Scholar 

  • Macy, R. W., Berntzen, A. K., Benz, M.: In vitro excystment of Sphaeridiotrema globulus. Metacercariae, structure of cyst, and the relationship to host specificity. J. Parasit. 54, 28–38 (1968)

    Google Scholar 

  • Oshima, T., Kihata, M.: Studies on the excystment of the metacercariae of Paragonimus westermani. I. Specially on the effect of bile salts. Bull. Inst. publ. Hlth (Tokyo) 7, 256–269 (1958a)

    Google Scholar 

  • Oshima, T., Yoshida, Y., Kihati, M.: Studies on excystment of metacercariae of Paragonimus westermani. 2. Influence of pepsin pretreatment on the effect of bile salts. Bull. Inst. publ. Hlth (Tokyo) 7, 270–274 (1958b)

    Google Scholar 

  • Rowan, W. B.: The mode of hatching of eggs of Fasciola hepatica. Exp. Parasit. 5, 118–137 (1956)

    Article  Google Scholar 

  • Rowan, W. B.: The mode of hatching of the eggs of Fasciola hepatica. II. Colloidal nature of the viscous cushion. Exp. Parasit. 6, 131–142 (1957)

    PubMed  Google Scholar 

  • Spector, W. S.: Handbook of biological data. Philadelphia: W. B. Saunders 1956

    Google Scholar 

  • Wilson, T. H.: Intestinal absorption. London: W. B. Saunders 1962

    Google Scholar 

  • Yokogawa, S., Cort, W. W., Yokogawa, M.: Paragonimus and paragonimiasis. Exp. Parasit. 10, 81–205 (1960)

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Asanji, M.F., Williams, M.O. Studies on the excystment of trematode metacercariae in vitro . Z. F. Parasitenkunde 47, 151–163 (1975). https://doi.org/10.1007/BF00382638

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00382638

Keywords

Navigation