Summary
A soluble fraction, derived from Naegleria fowleri trophozoites disrupted by freeze-thawing, was tested for antigenic properties. Intradermal injections of this preparation were administered to guinea pigs previously infected subcutaneously with viable N. fowleri. Delayed hypersensitivity to the antigen and loss of weight, the diagnostic symptom of visceral naegleriasis, were observed in the surviving animals. Fifty percent of the guinea pigs, however, did not lose weight and had a reduced reaction to the antigen. The apparent differences in the immunocompetence of guinea pigs inoculated subcutaneously and intranasally with N. fowleri are compared.
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Anderson, K., Jamieson, A.: Primary amoebic meningoencephalitis. Lancet 1972 I, 902–903
Apley, J., Clarke, S. K. R., Roome, A. P. C. H., Sandry, S. A., Saygi, G., Silk, B., Warhurst, D. C.: Primary amoebic meningoencephalitis in Britain. Brit. med. J. 1970 I, 596–599
Butt, C. G., Baro, C., Knorr, R. W.: Naegleria identified in amoebic meningoencephalitis. Amer. J. clin. Path. 50, 568–574 (1968)
Červa, L.: Experimental infection of laboratory animals by the pathogenic Naegleria gruberi strain VITEK. Folia parasit. (Praha) 18, 171–176 (1971)
Chang, S. L.: Etiological, pathological, epidemiological, and diagnostical consideration of primary amoebic meningoencephalitis. CRC Critical Rev. Microbiol. 2, 135–159 (1974)
Culbertson, C. G., Ensminger, P. W., Overton, W. M.: Pathogenic Naegleria sp. study of a strain isolated from human cerebral spinal fluid. J. Protozool. 15, 353–363 (1968)
Culbertson, C. G., Ensminger, P. W., Overton, W. M.: Amoebic cellulocutaneous invasion by Naegleria aerobia with generalized visceral lesions after subcutaneous injections. Amer. J. clin. Path. 57, 375–386 (1972)
Larson, C. L., Baker, R. E., Baker, M. B.: Comparison of the ability of protoplasm and culture filtrate (PPD) antigens from Mycobacterium tuberculosis, M. kansii, and M. batteyi to elicit delayed hypersensitivity reactions in infected guinea pigs. Amer. Rev. resp. Dis. 98, 1055–1057 (1968)
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This report is a part of a thesis presented by Peter Diffley in partial fulfillment of the requirements designated by the Department of Zoology, University of Montana, for the M.A. degree.
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Diffley, P., Skeels, M.R. & Sogandares-Bernal, F. Delayed type hypersensitivity in guinea pigs infected subcutaneously with Naegleria fowleri Carter. Z. F. Parasitenkunde 49, 133–137 (1976). https://doi.org/10.1007/BF00382420
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DOI: https://doi.org/10.1007/BF00382420