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Die Ultrastruktur der Trophozoiten und Merozoiten von Babesia herpailuri in Erythrozyten der Hauskatze nach Behandlung mit Imidocarb® (3,3′-bis-2-imidazolin-2-yl)-carbanilid

The ultrastructure of the intraerythrocytic stages of Babesia herpailuri-trophozoites and -merozoites after treatment with Imidocarb® (3,3′-bis-2-imidazolin-2-yl)-carbanilide

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Summary

The fine structure of trophozoites and especially of merozoites of Babesia herpailuri is described before and after treatment with Imidocarb® (Wellcome). The mostly piriform to oval merozoites possess an outer membrane and a supporting membrane below. The intratorium consists of a polar ring, rhoptries micronemes and the spherical body which lies beside the big nucleus and next to mitochondria. The endoplasmic reticulum and invaginations are not clearly formed.

The cellular changes of Babesia herpailuri, observed one hour after drug treatment in trophozoites and six hours later in merozoites, concern the form and function of the parasite: widening of the subpellicular endoplasmic reticulum and of the perinuclear space; sporadic dilatation of the endoplasmic reticulum of the merozoites (9 fig.). Damaged membranes, dissolution of the cellular membrane, disintegration of the nuclei as are known effects of the Berenil treatment to Babesia herpailuri, are not noted results after the Imidocarb treatment. The original membrane systems of trophozoites as well as of merozoites, remain unaffected by the drug as long as investigations were carried on (24 h).

The satisfying prophylactic effect of Imidocarb as well as the insignificant cellular damages on merozoites may be due to the small feeding of hemoglobin.

Zusammenfassung

Ziel der vorliegenden Arbeit ist die Darstellung der feinstrukturellen Veränderungen von Babesia herpailuri in Erythrozyten der Hauskatze nach Behandlung mit Imidocarb® (3,3′-bis-2-imidazolin-2-yl)-carbanilid. Im Zusammenhang mit der Frage nach der Wirkungsweise des Chemotherapeutikums bedurfte es der Untersuchung von Vorhandensein und Morphologie der Merozoiten des Parasiten. Die vorwiegend piriformen bis ovalen Merozoiten von Babesia herpailuri besitzen eine Elementarmembran und eine unter dieser liegende Stützmembran. Polring, Rhoptrien, Mikronemen und sphärischer Körper, der seitlich neben dem großen Kern und in der Nähe der Mitochondrien liegt, bilden das Intratorium. Endoplasmatisches Retikulum und Invaginationen sind zumeist schwach ausgebildet.

Die beobachteten Zellveränderungen an Babesia herpailuri (erstmals 60 min nach Therapiebeginn an den Trophozoiten und nach 6 h an den Merozoiten) betreffen Form und Funktion des Parasiten:

Ausweitung der Schläuche des subpellikulären endoplasmatischen Retikulums und des Kernspaltes der Trophozoiten; sporadische Erweiterung des ER im Zellinneren der Merozoiten. Membranschäden, Auflösung der Zellmembran oder der Zellkerne analog der Exposition von B. herpailuri in Berenil, erfolgen nach Imidocarb-Behandlung weder an Trophozoiten noch an Merozoiten. Das ursprüngliche Membransystem beider Parasitenstadien bleibt während des Verfolgungszeitraumes von 24 h erhalten. Der Wirkungsmechanismus des Imidocarb, auch hinsichtlich seiner prophylaktischen Wirkung, wird diskutiert.

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Authors and Affiliations

  1. Institut für Vergleichende Tropenmedizin und Parasitologie des Fachbereichs Tiermedizin der Universität München, Germany

    Edward Göbel & Hans Konrad Dennig

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  1. Edward Göbel
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  2. Hans Konrad Dennig
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Herrn Professor Dr. J. Bloch zum 60. Geburtstag gewidmet

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Göbel, E., Dennig, H.K. Die Ultrastruktur der Trophozoiten und Merozoiten von Babesia herpailuri in Erythrozyten der Hauskatze nach Behandlung mit Imidocarb® (3,3′-bis-2-imidazolin-2-yl)-carbanilid. Z. F. Parasitenkunde 49, 97–112 (1976). https://doi.org/10.1007/BF00382417

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  • DOI: https://doi.org/10.1007/BF00382417

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