Summary
High level lincomycin resistant strains of Escherichia coli were isolated and screened for altered ribosomal proteins and functions. Amongst 58 strains investigated by electrophoresis one had an altered ribosomal protein S7, another one a mutated L14 and two showed altered L15 proteins.
A correlation between these alterations and lincomycin resistant growth could not be demonstrated by genetic analysis for any of the mutants. In vitro, however, extracts from the two L15 mutants were less sensitive to inhibition by the drug. A gene locus (lin R) responsible for the lincomycin resistance phenotype was mapped at min 30 of the Escherichia coli chromosome near tyrR; it seems to be identical to the previously described linB locus (Apirion, 1967); however, in contrast to these reports it does not seem to alter any ribosomal function.
This is a preview of subscription content, log in via an institution to check access.
References
Apirion, D.: Three genes that affect Escherichia coli ribosomes. J. Mol. Biol. 30, 255–275 (1967)
Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bacteriol. Rev. 40, 116–167 (1976)
Celma, M.L., Monro, R.E., Vazquez, D.: Substrate and antibiotic binding sites at the peptidyl transferase centre of E. coli ribosomes. FEBS Lett. 6, 273–277 (1970)
Chang, F.N., Sih, C.J., Weisblum, B.: Lincomycin an inhibitor of aminoacyl sRNA binding to ribosomes. Proc. Natl. Acad. Sci. U.S.A. 55, 431–438 (1966)
Czernilofsky, A.P., Collatz, E.E., Stöffler, G., Kuechler, E.: Proteins at the tRNA binding sites of Escherichia coli ribosomes. Proc. Natl. Acad. Sci. U.S.A. 71, 230–234 (1974)
Igarashi, K., Ishitsuka, H., Akira, K.: Comparative studies on the mechanism of action of lincomycin, streptomycin, and erythromycin. Biochem. Biophys. Res. Commun. 37, 499–504 (1969)
Kaltschmidt, E., Wittmann, H.G.: Ribosomal proteins VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal. Biochem. 36, 401–412 (1970)
Miller, J.H.: Experiments in molecular genetics. Cold Spring Harbor Laboratory (1972)
Monro, R.E., Vazquez, D.: Ribosome-catalysed peptidyl transfer: Effects of some inhibitors of protein synthesis. J. Mol. Biol. 28, 161–165 (1967)
Nierhaus, K., Bordasch, K., Homann, H.E.: Ribosomal proteins XLIII. In vivo assembly of Escherichia coli ribosomal proteins. j. Mol. Biol. 74, 578–597 (1973)
Pestka, S.: Inhibitors of protein synthesis. In: Molecular mechanisms of protein biosynthesis (H. Weissbach and S. Pestka, eds.), pp. 467–553. New York: Acad. Press 1977
Teraoka, H., Nierhaus, K.H.: Das Antibiotikum Erythromycin stoppt Ribosomen-Aktivität. Umschau 77, 347–348 (1977)
Teraoka, H., Tanaka, K., Tamaki, M.: The comparative study on the effects of chloramphenicol, erythromycin and lincomycin on polylysine synthesis in an Escherichia coli cell-free system. Biochim. Biophys. Acta 174, 776–778 (1969)
Vazquez, D.: Antibiotics affecting chloramphenicol uptake by bacteria. Their effect on amino acid incorporation in a cell free system. Biochim. Biophys. Acta 114, 289–295 (1966)
Vazquez, D.: Inhibitors of protein synthesis. FEBS Letters 40, Suppl. 63–84 (1974)
Wallace, B.J., Pittard, J.: Regulator gene controlling enzymes concerned in tyrosine biosynthesis in Escherichia coli. J. Bacteriol. 97, 1234–1241 (1969)
Author information
Authors and Affiliations
Additional information
Communicated by H.G. Wittmann
Rights and permissions
About this article
Cite this article
Hummel, H., Piepersberg, W. & Böck, A. Analysis of lincomycin resistance mutations in Escherichia coli . Molec. Gen. Genet. 169, 345–347 (1979). https://doi.org/10.1007/BF00382280
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00382280