Summary
Centrifugal elutriation was used to separate cells in different stages of the cell cycle from a culture of Saccharomyces cerevisiae in balanced exponential growth. The rate of DNA and RNA synthesis was determined using a pulse-long-term label technique that is capable of distinguishing between exponential, linear, and periodic variations in the rate of synthesis through the cell cycle. It was found that while the rate of DNA synthesis varies periodically through the cell cycle, the rate of synthesis of mRNA, rRNA, and tRNA increases exponentially through the cell cycle. The implications of these findings for the control of RNA synthesis are discussed.
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References
Campbell, A.: Synchronization of cell division. Bacteriol. Rev. 21, 263–272 (1957)
Elliott, S.G., McLaughlin, C.S.: The rate of macromolecular synthesis through the cell cycle of the yeast Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA, 75, 4384–4388 (1978)
Fraser, R.S.S., Carter, B.L.A.: Synthesis of polyadenylated mRNA during the cell cycle of Saccharomyces cerevisiae. J. Mol. Biol. 104, 223–242 (1976)
Fraser, R.S.S., Moreno, F.: Rates of synthesis of poly (A) mRNA and rRNA during the cell cycle of Schizosaccharomyces pombe. J. Cell Sci. 21, 497–521 (1976)
Gordon, C.N., Elliott, S.G.: Fractionation of Saccharomyces cerevisiae cell populations by centrifugal elutriation. J. Bacteriol. 129, 97–100 (1977)
Hartwell, L.H.: The Saccharomyces cerevisiae cell cycle. Bacteriol. Rev. 38, 168–198 (1974)
Hartwell, L.H., Culotti, J., Pringle, J., Reid, B.: Genetic control of the cell division cycle in yeast. Science 183, 46–51 (1974)
Hynes, N., Phillips, S.: Rate of synthesis of polyadenylate containing RNA during the yeast cell cycle. J. Bacteriol. 128, 502–505 (1976)
Loening, U.E.: The fractionation of high-molecular weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J. 102, 251–257 (1967)
Mitchison, M.: The biology of the cell cycle. I. Cambridge, United Kingdom: Cambridge University Press 1971
Petersen, N.S., McLaughlin, C.S., Nierlich, D.P.: Half-life of yeast messenger RNA. Nature 260, 70–72 (1976)
Sebastian, J., Carter, B.L.A., Halvorson, H.O.: Use of yeast populations fractionated by zonal centrifugation to study the cell cycle. J. Bacteriol. 108, 1045–1050 (1971)
Stringer, J.R., Holland, L.E., Swanstrom, R.I., Pivo, K., Wagner, E.K.: Quantitation of herpes simplex virus type 1. RNA in infected HeLa cells. J. Virol. 21, 889–901 (1977)
Sogin, S.J., Carter, B.L.A., Halvorson, H.O.: Changes in the rate of ribosomal RNA synthesis during the cell cycle of Saccharomyces cerevisiae. Exp. Cell Res. 89, 127–138 (1974)
Tauro, P., Schweizer, E., Epstein, R., Halvorson, H.O.: Synthesis of macromolecules during the cell cycle in yeast. In: The cell cycle. Gene-enzyme interactions. 1 New York: Academic Press 1969
Williamson, D.H.: The timing of deoxyribonucleic acid synthesis in the cell cycle of Saccharomyces cerevisiae. J. Cell Biol. 25, 517–528 (1965)
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Communicated by W. Gajewski
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Elliott, S.G., McLaughlin, C.S. Regulation of RNA synthesis in yeast III. Molec. Gen. Genet. 169, 237–243 (1979). https://doi.org/10.1007/BF00382269
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DOI: https://doi.org/10.1007/BF00382269