Summary
A high performance liquid chromatographic method for the determination of phenylsulfate and phenylglucuronide, metabolites of benzene, or phenol in urine is described. For the determination of urinary phenylsulfate, a stainless-steel column packed with octadecyl-silanized silica gel was used, and the mobile phase was mixed with a solution of methanol/water acetic acid (20/80/0.2) containing 0.05 M tetra-n-butylammonium bromide. For the exact determination of urinary phenylglucuronide, a stainless-steel column packed with the same resin was used and the mobile phase was a solution of methanol/water/acetic acid (50/50/0.2) containing 0.05 M tetra-n-butylammonium bromide. The method is simple and specific. Urine can be analyzed directly, without solvent extraction or pretreatment. The method has a lowest detection limit of 0.2 μg on the column. The whole analysis and quantitative determination can be performed within about 15 min for samples containing phenylsulfate and phenylglucuronide in the urine of rats.
The phenylsulfate excreted was equivalent to 36.1% and phenylglucuronide was 51.8% of phenol administered to rats intraperitoneally.
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References
Bertolacini, R. J., Barney, J. E.: Colorimetric determination of sulfate with barium chloranilate. Anal. Chem. 29, 281–283 (1957)
Deichmann, W. B., Keplinger, M. L.: Industrial hygiene and toxicology. In: Phenol 1963–1975, Patty, F. D. (ed.). Interscience Publishers 1962
Fischman, W. H., Green, S.: Microanalysis of glucuronide glucuronic acid as applied to β-glucuronidase and glucuronic acid studies. J. Biol. Chem. 215, 527–537 (1955)
Folin, O., Ciocalteu, V.: On tyrosine and tryptophan determinations in proteins. J. Biol. Chem. 73, 627–650 (1927)
Gerade, H. W.: Toxicology and biochemistry of aromatic hydrocarbons, pp. 97–108. London, New York, Princeton: Elsevier 1960
Nomiyama, K., Nomiyama, H.: Gas-liquid chromatographic determination of benzene metabolites. J. Chromatogr. 44, 386–388 (1969)
Ohtsuji, H., Ikeda, M.: A rapid colorimetric method for the determination of phenylglyoxylic and mandelic acids. Br. J. Ind. Med. 27, 150–154 (1970)
Ohtsuji, H., Ikeda, M.: Quantitative relationship between atmospheric phenol vapour and phenol in the urine of workers in bakelite factories. Br. J. Ind. Med. 29, 70–73 (1972)
Parks, D. V., Williams, R. T.: Studies in detoxication. Biochem. J. 55, 337–340 (1953)
Porteus, J. W., Williams, R. T.: Studies in detoxication. Biochem. J. 44, 46–55 (1949)
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Ogata, M., Yamasaki, Y. High performance liquid chromatography for the quantitative determination of urinary phenylsulfate and phenylglucuronide as indices of benzene and phenol exposure in rats. Int. Arch Occup Environ Heath 44, 177–184 (1979). https://doi.org/10.1007/BF00381132
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DOI: https://doi.org/10.1007/BF00381132