Abstract
The ability of isolated pea-shoot mitochondria conditioned to incorporate ammonia into glutamate to reassimilate endogenously produced ammonia from glycine transformation was investigated. In the presence of 1 mM to 20 mM glycine less than 15% of the ammonia liberated was found to be incorporated into glutamate. Thus, a prominent role of mitochondrial glutamate dehydrogenase in the reassimilation of intramitochondrially produced ammonia can be excluded.
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Abbreviations
- GDH:
-
Glutamate dehydrogenase (L-glutamate: NAD+ oxidoreductase (deaminating), EC 1.4.1.2)
References
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Hartmann, T., Ehmke, A. Role of mitochondrial glutamate dehydrogenase in the reassimilation of ammonia produced by glycine serine transformation. Planta 149, 207–208 (1980). https://doi.org/10.1007/BF00380885
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DOI: https://doi.org/10.1007/BF00380885