Abstract
In the leaves of rye seedlings (Secale cereale L.) grown at an elevated temperature of 32°C the formation of plastidic 70S ribosomes is specifically prevented. The resulting plastid ribosome-deficient leaves, which are chlorotic in light, represent a system for the identification of translation products of the 80S ribosomes among the chloroplastic proteins. Searching for the primary heat-sensitive event causing the 70S ribosome-deficiency, the thermostability of the chloroplastic capacity for RNA synthesis was investigated. The RNA polymerase activity of isolated normal chloroplasts from 22°-grown rye leaves was not inactivated in vitro at temperatures between 30° and 40°C. The ribosome-deficient plastids purified from bleached 32°-grown leaf parts contained significant RNA polymerase activity which was, however, lower than in functional chloroplasts. After application of [3H]uridine to intact leaf tissues [3H]uridine incorporation was found in ribosome-deficient plastids of 32°C-grown leaves. The amount of incorporation was similar to that in the control chloroplasts from 22°C-grown leaves. According to these results, it is unlikely that the non-permissive temperature (32°C) causes a general inactivation of the chloroplastic RNA synthesis in rye leaves.
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References
Bedbrook, J.R., Kolodner, R. (1979) The structure of chloroplast DNA. Annu. Rev. Plant Physiol. 30, 593–620
Bogorad, L. (1975) Evolution of organelles and eukaryotic genomes. Science 188, 891–898
Bohnert, H.J., Driesel, A.J., Herrmann, R.G. (1976) Characterization of the RNA compounds synthesized by isolated chloroplasts. In: Genetics and biogenesis of chloroplasts and mitochondria, pp. 629–636, Bücher, Th., Neupert, W., Sebald, W., Werner, S eds. North Holland Publ. Co., Amsterdam
Boynton, J.E., Gillham, N.W., Harris, E.H., Tingle, C.L., Winkle-Swift, K. van, Adams, G.M.W. (1976) Transmission, segregation and recombination of chloroplast genes in Chlamydomonas. In: Genetics and biogenesis of chloroplasts and mitochondria, pp. 313–322, Bücher, Th., Neupert, W., Sebald, W., Werner, S., eds. North Holland Publ. Co. Amsterdam
Bottomley, W. (1970), Deoxyribonucleic acid-dependent ribonucleic acid polymerase activity of nuclei and plastids from etiolated peas and their response to red and far red light in vivo. Plant Physiol. 45, 608–611
Bottomley, W., Smith, H.J., Bogorad, L. (1971) RNA polymerases of maize. Partial purification and properties of the chloroplast enzyme. Proc. Natl. Acad. Sci. USA 68, 2412–2416
Bottomley, W., Whitfeld, P.R., Spencer, L. (1972) Stimulation of chloroplast DNA-dependent RNA polymerase by exogenous DNA. Arch. Biochem Biophys 151, 35–41
Brandt, P., Wiessner, W. (1977) Unterschiedliche, Temperaturoptima der DNA-abhängigen RNA-Polymerasen von Euglena gracilis, Stamm Z und ihre Bedeutung für die experimentelle Erzeugung der permanenten Apochlorose durch höhere Temperatur. Z. Pflanzenphysiol. 85, 53–60
Cohen, D., Schiff, J.A. (1976) Events surrounding the early development of Euglena chloroplasts. Photoregulation of the transcription of chloroplastic and cytoplasmic ribosomal RNAs. Arch. Biochem. Biophys. 177, 201–216
Edelman, M., Schiff, J.A., Epstein, H.T. (1965) Studies of chloroplast development in Euglena. XII. Two types of satellite DNA. J. Mol. Biol. 11, 769–774
Ellis, R.J. (1976) Protein and nucleic acid synthesis by chloroplasts. In: The Intact Chloroplast, pp. 335–364, J. Barber, ed. Elsevier, North Holland Biomedical Press
Ellis, R.J., Hartley, M.R. (1971) Sites of synthesis of chloroplast proteins. Nature (London) New Biol. 233, 193–196
Feierabend, J. (1977) Capacity for chlorophyll synthesis in heatbeleached 70S ribosome-deficient rye leaves. Planta 135, 83–88
Feierabend, J. (1979) Role of cytoplasmic protein synthesis and its coordination with the plastidic protein synthesis in the biogenesis of chloroplasts. Ber. Dtsch. Bot. Ges. 92, 553–574
Feierabend, J., Beevers, H. (1972) Developmental studies on microbodies in wheat leaves. II. Ontogeny of particulate enzyme associations. Plant Physiol. 49, 33–39
Feierabend, J., Mikus, M. (1977) Occurrence of a high temperature sensitivity of chloroplast ribosome formation in several higher plants. Plant Physiol. 59, 863–867
Feierabend, J., Schrader-Reichhardt, U. (1976) Biochemical differentiation of plastids and other organelles in rye leaves with a high-temperature-induced deficiency of plastid ribosomes. Planta 129, 133–145
Feierabend, J., Wildner, G. (1978) Formation of the small subunit in the absence of the large subunit of ribulose 1,5-bisphosphate carboxylase in 70S ribosome-deficient rye leaves. Arch. Biochem. Biophys 186 283–291
Geyl, D., Böck, A., Wittmann H.G. (1977) Cold-sensitive growth of a mutant of Escherichia coli with an, altered ribosomal protein S8: Analysis of revertants. Mol. Gen Genet. 152, 331–336
Harris, E.H., Boynton, J.E., Gillham, N.W., Tingle, C.L., Fox, S.B. (1977) Mapping of chloroplast genes involved in chloroplast ribosome biogenesis in Chlamydomonas reinhardtii. Mol. Gen. Genet. 155, 249–265
Hartley, M.R., Ellis, R.J. (1973) Ribonucleic acid synthesis in chloroplasts. Biochem. J. 134, 249–262
Herrmann, R.G., Feierabend, J. (1980) The presence of DNA in ribosome-deficient plastids of heat-bleached rye leaves. Eur. J. Biochem. 104, 603–609
Isono, K., Cumberlidge, A.G., Isono, S., Hirota, Y. (1977) Further temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins. Mol. Gen. Genet. 152, 239–243
Kowallik, K.V., Herrmann, R.G. (1977) General and molecular cytology: Chloroplasts. Prog. Bot. 39, 1–17
Parish, J.H., Kirby, K.S. (1966) Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochim. Biophys. Acta 129, 554–562
Pringsheim, E.G., Pringsheim O. (1952) Experimental elimination of chromatophores and eye-spot in Euglena gracilis. New Phytol. 51, 65–76
Ray, D.S., Hanawalt, P.C. (1965) Satellite DNA components in Euglena gracilis cells lacking chloroplasts. J. Mol. Biol. 11, 760–768
Schäfers, H.-A., Feierabend, J. (1976) Ultrastructural differentiation of plastids and other organelles in rye leaves with a high-temperature-induced deficiency of plastid ribosomes. Cytobiologie 14, 75–90
Schiemann, J., Wollgiehn, R., Parthier, B. (1978) DNA-dependent RNA polymerase in Euglena gracilis broken chloroplasts. Biochem. Physiol Pflanz. 172, 507–519
Smith, H.J., Bogorad, L. (1974) The polypeptide subunit structure of the DNA-dependent RNA polymerase of Zea mays chloroplasts. Proc. Natl. Acad. Sci. USA 71, 4839–4842
Surzucki, S.J., Goodenough, U.W., Levine, R.P., Armstrong, J.J. (1970) Nuclear and chloroplast control of chloroplast structure and function in Chlamydomonas reinhardi. In: Control of organelle development. Symp. Soc. Exp. Biol. XXIV, pp. 13–33, Miller, P.L., ed. University Press, Cambridge
Whatley, F.R., Arnon, D.J. (1963) Photosynthetic phosphorylation in plants. In: Methods in enzymology 6, pp. 308–313, Colowick, S.P., Kaplan, N.O., eds Academic Press, New York
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Bünger, W., Feierabend, J. Capacity for RNA synthesis in 70S ribosome-deficient plastids of heat-bleached rye leaves. Planta 149, 163–169 (1980). https://doi.org/10.1007/BF00380878
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DOI: https://doi.org/10.1007/BF00380878