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Phloem loading in Vicia faba leaves: Effect of N-ethylmaleimide and parachloromercuribenzenesulfonic acid on H+ extrusion, K+ and sucrose uptake

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Abstract

The effects of a penetrating (NEM) and a non-penetrating (PCMBS) sulfhydryl-specific reagent on proton extrusion, 86Rb and [U-14C]sucrose uptake by Vicia faba leaves have been studied. Proton extrusion was strongly or completely inhibited by 0.1 mM NEM. 86Rb and [U-14C]sucrose uptake were markedly reduced by NEM concentrations equal to or higher than 0.5 mM. Under our experimental conditions, PCMBS (1 mM) exerted a strong inhibition on [14C]sucrose uptake but did not inhibit proton extrusion and 86Rb uptake. The sensitivity of phloem loading to PCMBS is thought to be a consequence of sugar-carrier blockage and not of inhibition of the proton pump.

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Abbreviations

CCCP:

carbonylcyanide-m-chlorophenylhydrazone

DES:

diethylstilbestrol

DCCD:

dicyclohexylcarbodiimide

FC:

Fusicoccin

NEM:

N-ethylmaleimide

PCMBS:

p-chloromercuribenzenesulfonic acid

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Authors and Affiliations

  1. Laboratoire de Biologie et Physiologie Végétales, Université de Poitiers, 25 Faubourg Saint Cyprien, F-86000, Poitiers, France

    Serge Delrot, Jean-Pierre Despeghel & Jean-Louis Bonnemain

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  1. Serge Delrot
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  2. Jean-Pierre Despeghel
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  3. Jean-Louis Bonnemain
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Delrot, S., Despeghel, JP. & Bonnemain, JL. Phloem loading in Vicia faba leaves: Effect of N-ethylmaleimide and parachloromercuribenzenesulfonic acid on H+ extrusion, K+ and sucrose uptake. Planta 149, 144–148 (1980). https://doi.org/10.1007/BF00380875

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  • DOI: https://doi.org/10.1007/BF00380875

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