Abstract
Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in the resultant plants. Shoots and plants obtained from different lines and different regeneration events differed morphologically and genetically. Most regenerants show morphological abnormalities and unusual organizational patterns. Flowering forms have so far been sterile. “Asymmetric” hybrids (i.e., hybrids bearing most genetic material of one of the parent species and only few chromosomes of the other) were more regular in morphology. The results represent the first case of intergeneric-intertribal hybridization of flowering plants.
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Barthlott, W., Ehler, N.: Rasterelektronenmikroskopie der Epidermisoberflächen von Spermatophyten. Wiesbaden: Franz-Steiner 1977
Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158 (1968)
Gleba, Y.: Extranuclear inheritance investigated by somatic hybridization. In: Frontiers of plant tissue culture 1978, pp. 95–102. Thorpe, T.A., ed. Calgary: University Press 1978
Gleba, Y., Hoffmann, F.: Hybrid cell lines Arabidopsis thaliana+Brassica campestris: no evidence for specific chromosome elimination. Mol. Gen. Genet. 165, 257–264 (1978)
Gleba, Y., Butenko, R.G., Sytnik, K.M.: Protoplast fusion and parasexual hybridization in Nicotiana tabacum. Dokl. Akad. Nauk SSSR 221, 1196–1198 (1975)
Gleba, Y., Kohlenbach, H.W., Hoffmann, F.: Root morphogenesis in somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris. Naturwissenschaften 65, 655 (1978)
Krumbiegel, G., Schieder, O.: Selection of somatic hybrids after fusion of protoplasts from Datura innoxia and Atropa belladonna. Planta 145, 371–375 (1979)
Gosch, G., Reinert, J.: Cytological identification of colony formation of intergeneric somatic hybrid cells. Protoplasma 96, 23–38 (1978)
Maliga, P., Lázár, G., Joó, F., H.-Nagy, A., Menczel, L.: Restoration of morphogenetic potential in Nicotiana by somatic hybridisation. Mol. Gen. Genet. 157, 291–296 (1977)
Melchers, G., Sacristán, M.D., Holder, A.A.: Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res. Commun. 43, 203–218 (1978)
Pontecorvo, G.: Induction of directional chromosome elimination in somatic cell hybrids. Nature (London) 230, 367–369 (1971)
Schenck, H., Hoffmann, F.: Callus and root regeneration from mesophyll protoplasts of basic Brassica species: B. campestris, B. oleracea and B. nigra. Z. Pflanzenzücht. 82, 354–360 (1979)
Vaughan, J.G., Mac Leod, A.J., Jones, B.M.G.: The biology and chemistry of the cruciferael London: Academic Press 1976
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Dedicated to the memory of G.D. Karpechenko, author of the theory of remote hybridization and creator of Raphanobrassica hybrid (1928)
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Gleba, Y.Y., Hoffmann, F. “Arabidobrassica”: A novel plant obtained by protoplast fusion. Planta 149, 112–117 (1980). https://doi.org/10.1007/BF00380870
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DOI: https://doi.org/10.1007/BF00380870