Abstract
Coniferin specific- and isoflavone 7-glucoside specific β-glucosidases have been localized in stem and root sections of chick pea (Cicer arietinum L.) seedlings by the indirect immunofluorometrical method. The coniferin specific β-glucosidase has been found in the cell walls of the tracheary elements and of the endo-, epi-, and exodermis. All these tissues are known to contain either lignin or polymers, like suberin and cutin, which consist partially of phenylpropanoid elements. The localization of this β-glucosidase is therefore in agreement with its postulated relationship to the phenylpropanoid metabolism. The isoflavone 7-glucoside specific β-glucosidase, on the other hand, is predominantly located in the parenchymatic cortex cells, and obviously in the cytoplasm. These cells are known to contain the isoflavone formononetin, which has been shown to undergo turnover in chick pea seedlings. We therefore have good reason to assume that this β-glucosidase is involved in the metabolism of the 7-glucoside of this isoflavone.
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Abbreviations
- SDS:
-
sodium dodecylsulfate
- PBS:
-
physiological phosphate saline
References
Amrhein, N., Diederich, E. (1980) Turnover of isoflavones in Cicer arietinum L. Naturwissenschaften 67, 40–41
Axen, R., Porath, U., Ernback, S (1967) Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature (London) 214, 1302–1304
Barz, W. (1969) Über den Umsatz von Isoflavonen und Cumöstanen in Cicer arietinum L. und Phaseolus aureus Roxb. Z. Naturforsch. 24b, 234–239
Barz, W., Hösel, W. (1971) Über den Umsatz von Flavonolen und Isoflavonen in Cicer arietinum L. Phytochemistry 10, 335–341
Baumgartner, B., Matile, Ph. (1976) Immunocytochemical localization of acid ribonuclease in morning glory flower tissue. Biochem. Physiol. Pflanzen 170, 279–285
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254
Braune, W., Leman, A., Taubert, H. (1967) Pflanzenanatomisches Praktikum. p. 298 and 304, Gustav Fischer Verlag, Jena
Burmeister, G., Hösel W. (1980) Comparison of the β-glucosidases from Cicer arietinum L. cell cultures and whole seedlings. Planta Med. 40, 40–48
Clarke, A.E., Knox, R.B., Hermyn, M.A. (1975) Localization of lectins in legume cotyledons. J. Cell Sci. 19, 157–167
Freudenberg, K., Harkin, J.M. (1963) The glucosides of cambial sap of spruce. Phytochemistry 2, 189–193
Freudenberg, K., Reznik, H., Fuchs, W., Reichert, M. (1955) Untersuchungen über die Entstehung des Lignins und des Holzes. Naturwissenschaften 42, 29–35
Gierse, H.-D., Barz, W. (1976) Ein fluoreszenzmikroskopischer Nachweis von β-Glucosidase in Wurzeln von Cicer arietinum L. Protoplasma 88, 1–6
Hapner, S.J., Hapner, K.D. (1978) Rhodamine immunohistofluorescence applied to plant tissue. Histochem. Cytochem. 26, 478–482
Harboe, N., Ingild, A. (1973) A manual of quantitative immunelectrophoresis. Vol. 1, Suppl. 2, Chapter 23 Universitetforlaget, Oslo
Hattersley, P.W., Watson, L., Osmond, C.B. (1977) In situ immunofluorescent labelling of ribulose-1,5-bisphosphate carboxylase in leaves of C3 and C4 plants. Aust. J. Plant Physiol. 4, 523–539
Hösel, W. (1976) Reinigung und Charakterisierung zweier β-Glykosidasen mit bevorzugter Spezifität für Biochanin A-7-β-apiosylglucosid aus Cicer arietinum L. Hoppe-Seyler's Z. Physiol. Chem. 357, 1673–1681
Hösel, W., Barz, W. (1975) β-Glucosidases from Cicer arietinum L. Eur. J. Biochem. 57, 607–616
Hösel, W., Borgmann, E. (1978) Development of lignin biosynthetic enzymes during lignification of Petroselinum hortense cell cultures. 12th FEBS Meeting, Dresden, GDR; Abstract No. 308
Hösel, W., Todenhagen, R. (1980) Characterization of a β-glucosidase from Glycine max. which hydrolyses coniferin and syringin. Phytochemistry 19, 1349–1353
Hösel, W., Surholt, E., Borgmann, E. (1978) Characterization of β-glucosidase isoenzymes possibly involved in lignification from chick pea (Cicer arietinum L.) cell suspension cultures. Eur. J. Biochem. 84, 487–492
Hudson, L., Hay, F.W. (1976) In: Practical immunology. pp. 192 Blackwell Scientific Publ., Oxford
Kanfer, J.N., Petrovich, G., Mumford, R.A. (1973) Purification of α- and β-galactosidases by affinity chromatography. Anal. Biochem. 55, 301–305
Kanfer, J.N., Mumford, R.A., Rhagavan, S.S., Byrd, J. (1974) Purification of β-glucosidase activities from bovine spleen by affinity chromatography. Anal. Biochem. 60, 200–205
Knox, R.B. (1971) Pollen-wall protein: localization, enzymic and antigenic activity during development in Gladiolus (Iridaceae). J. Cell Sci. 9, 209–237
Koblitz, H. (1961) Einige Bemerkungen zum histochemischen Nachweis von an der Verholzung beteiligten Fermenten. Ber. Dtsch. Bot. Ges. 74, 274–287
Kolattukudy, P.E. (1980) Cutin, suberin and waxes. In: The biochemistry of plants: a comprehensive treatise. P.K. Stumpf, E.E. Conn, eds. Vol. 4, pp. 571–645; Academic Press, New York
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275
Marcinowski, S., Grisebach, H. (1977) Turnover of coniferin in spruce seedlings. Phytochemistry 16, 1665–1667
Marcinowski, S., Grisebach, H. (1978) Enzymology of lignification. Cellwall-bound β-glucosidases from spruce (Picea abies) seedlings. Eur. J. Biochem. 87, 37–44
Marcinowski, S., Falk, H., Hammer, D.K., Hoyer, B., Grisebach, H. (1979) Appearance and localization of a β-glucosidase hydrolyzing coniferin in spruce (Picea abies) seedlings. Planta 144, 161–165
Murray, D.R., Knox, R.B. (1977) Immunofluorescent localization of urease in the cotyledons of jack bean, Canavalia ensiformis. J. Cell Sci. 26, 9–18
Reznik, H. (1955) Über den histochemischen Nachweis der an der Verholzung beteiligten β-Glucosidasen. Planta 45, 455–469
Sarkanen, K.V., Ludwig, C.H. (1971) Lignins. pp. 28–33, 109–111 Wiley Interscience, New York
Surholt, E. (1979) Beteiligung von β-Glucosidasen am Sekundärstoffwechsel von höheren Pflanzen. Doctoral thesis, University of Münster, FRG
Surholt, E., Hösel, W. (1981) Identification of coniferin specific β-glucosidases in Cicer arietinum L. seedlings. Z. Pflanzenphysiol. 101, 207–214
Wong, E. (1975) The isoflavonoids, In: The flavonoids pp. 743–800. J.B. Harbone, T.J. Mabry, H. Mabry eds. Chapman and Hall, London
Zwilling, R. (1977) Immunologisches Praktikum. Gustav Fischer Verlag, Stuttgart New York
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The results are part of the thesis of Gerd Burmeister, 1980, University of Münster
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Burmeister, G., Hösel, W. Immunohistochemical localization of β-glucosidases in lignin and isoflavone metabolism in Cicer arietinum L. seedlings. Planta 152, 578–586 (1981). https://doi.org/10.1007/BF00380831
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DOI: https://doi.org/10.1007/BF00380831