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β-Glucan synthetase activity in Golgi vesicles ofPetunia hybrida

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Abstract

β-Glucan synthetase activity has been demonstrated in a Golgi vesicle fraction isolated from pollen tubes ofPetunia hybrida. Thisβ-glucan synthetase activity differs from that of most other higher plants in its inability to incorporate [14C]glucose from GDP-[14C]glucose. UDP-[14C]glucose, however, is an appropriate glucose donor for this enzyme. The optimum conditions for thisβ-glucan synthetase activity are: 1 mg Golgi vesicle protein/ml reaction mixture; pH=±8 and a temperature of 25°C. The newly synthesized alkali-insoluble glucan containsβ-1,3- as well as β-1,4-glucosidic linkages.

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Authors and Affiliations

  1. Department of Botany, University of Nijmegen, Toernooiveld, Nijmegen, The Netherlands

    J. P. F. G. Helsper & M. M. A. Sassen

  2. Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands

    J. H. Veerkamp

Authors
  1. J. P. F. G. Helsper
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  2. J. H. Veerkamp
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  3. M. M. A. Sassen
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Helsper, J.P.F.G., Veerkamp, J.H. & Sassen, M.M.A. β-Glucan synthetase activity in Golgi vesicles ofPetunia hybrida . Planta 133, 303–308 (1977). https://doi.org/10.1007/BF00380693

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  • DOI: https://doi.org/10.1007/BF00380693

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