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The effect of temperature on glycollate decarboxylation in leaf peroxisomes

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Abstract

[1-14C]glycollate was oxidised to14CO2 by peroxisomes isolated from leaves of spinach beet about 3 times as rapidly at 35°C as at 25°C; the rate was further increased with rise in temperature to a maximum at 55°C. These increases are shown to be mainly due to the increased H2O2 available to oxidise glyoxylate non-enzymically as a result of the higher temperature coefficient of glycollate oxidase activity relative to that of catalase. These results are compared with similar increases in the rate of14CO2 release between 25°C and 35°C when [1-14C]glycollate was supplied to leaf discs in light or darkness. The role of these reactions in accounting for the temperature effect on the release of photorespiratory CO2 is discussed.

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Abbreviations

PHMS:

Pyrid-2-yl-α-hydroxymethane sulphonate

FMN:

flavin mononucleotide

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Author notes

  1. B. Grodzinski

    Present address: Botany School, Cambridge University, Downing Street, CB2 3EA, Cambridge, UK

Authors and Affiliations

  1. Botany School, Oxtord University, South Parks Road, OXI 3RA, Oxford, UK

    B. Grodzinski & V. S. Butt

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  1. B. Grodzinski
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  2. V. S. Butt
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Grodzinski, B., Butt, V.S. The effect of temperature on glycollate decarboxylation in leaf peroxisomes. Planta 133, 261–266 (1977). https://doi.org/10.1007/BF00380687

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  • DOI: https://doi.org/10.1007/BF00380687

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