Abstract
The non-photosynthetic chromoplast membranes from the corona ofNarcissus pseudonarcissus L. were investigated for their lipid synthetic capabilities. The following activities were detected: galactosylation of diacylglycerol and galactosydiacylglycerols, glycosylation of sterols, acylation of monogalactosyldiacylglycerol and steryl glycosides from an unknown endogenous donor, acylation of phospholipids from acyl-CoA, and acylation of phosphatidyl inositol from phosphatidyl choline. Furthermore, activities of an acyl thioesterase, a sugar epimerase, and a phospholipase A2 were measured.
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Abbreviations
- MGDG:
-
monogalactosyldiacylglycerol
- DGDG:
-
digalactosyldiacylglycerol
- TGDG:
-
tri-and tetragalactosyldiacylglycerol
- SG:
-
steryl glycoside
- SL:
-
sulfolipid
- ACP:
-
acyl carrier protein
References
Axelos, M., Péaud-Lenoel, C.: Identification de l'activité phosphatidyléthanolamine: stérolglucoside transacylase extraite du blé. C.R. Acad. Sc. Paris. Ser. D237, 1434–1437 (1971)
Beeler, D.A., Porter, J.W.: The enzymatic conversion of phytoene to phytofluene. Biochem. biophys. Res. Commun.8, 367–371 (1962)
Charlton, J., Treharne, K.J., Goodwin, T.W.: Incorporation of [2-14C]mevalonic acid into phytoene by isolated chloroplasts. Biochem. J.105, 205–208 (1967)
Clark, B.R., Rubin, R.T., Arthur, R.G.: A new micro method for determination of cholesterol in serum. An. Biochem.24, 27–33 (1968)
Decker, K., Uehleke, H.: Eine enzymatische Isomerisierung von Lycopin undβ-Carotin. Hoppe-Seyler Z. physiol. Chem.323, 61–71 (1961)
Douce, R.: Sites of biosynthesis of galactolipids in spinach chloroplasts. Science183, 852–853 (1974)
Eichenberger, W., Grob, E.C.: Enzymatic formation of steryl glycosides by homogenates and chloroplast preparations from lettuce and spinach leaves. In: Progress in Photosynthesis Research (H. Metzner, ed.) Vol. I, pp. 338–344. München: C. Lichtenstern 1969
Eichenberger, W., Grob, E.C.: Enzymatische Acylierung von Sterin-14C-glucosid durch ein lösliches Enzym aus Karotten. Chimia24, 394–396 (1970)
Eichenberger, W., Menke, W.: Sterole in Blättern und Chloroplasten. Z. Naturforsch.21b, 859–867 (1966)
Eichenberger, W., Newman, D.W.: Hexose transfer from UDP-hexose in the formation of steryl glycosides and esterified steryl glycosides in leaves. Biochem. biophys. Res. Commun.32, 366–374 (1968)
Falk, H., Liedvogel, B., Sitte, P.: Circular DNA in isolated chromoplasts. Z. Naturforsch.29c, 541–544 (1974)
Forsee, W.T., Laine, R.A., Elbein, A.D.: Solubilization of a particulate UDP-glucose: sterolβ-glucosyltransferase in developing cotton fibers and seeds and characterization of steryl-6-acyl-D-glucosides. Arch. Biochem.161, 248–259 (1974)
Hill, H.M., Calderwood, S.K., Rogers, L.J.: Conversion of lycopene toβ-carotene by plastids isolated from higher plants. Phytochem.10, 2051–2058 (1971)
Joyard, J., Douce, R.: Mise en evidence et role des diacylglyerols de l'envelope des chloroplastes d'épinard. Biochim. Biophys. Acta424, 125–131 (1976)
Kannangara, C.G., Stumpf, P.K.: Fat metabolism in higher plants L. The biosynthesis of polyunsaturated fatty acids by isolated spinach chloroplasts. Arch. Biochem.148, 414–424 (1972)
Kannangara, C.G., Jacobson, B.S., Stumpf, P.K.: Fat metabolism in higher plants LVII. A comparison of fatty acid synthesizing enzymes in chloroplasts isolated from mature and immature leaves of spinach. Plant Physiol52, 156–161 (1973)
Kushwaha, S.C., Subbarayan, C., Beeler, D.E., Porter, J.W.: The conversion of lycopene-15,15′-3H to cyclic carotenes by soluble extracts of higher plant plastids. J. Biol. Chem.244, 3635–3642 (1969)
Liedvogel, B., Kleinig, H.: Galactolipid synthesis in chromoplast internal membranes of the daffodil. Planta (Berl.)129, 19–21 (1976)
Liedvogel, B., Sitte, P., Falk, H.: Chromoplasts in the daffodil: fine structure and chemistry. Cytobiol.12, 155–174 (1976)
Marshall, S.O., Kates, M.: Biosynthesis of phosphatidyl glycerol by cell-free preparations from spinach leaves. Biochim. Biophys. Acta260, 558–570 (1972)
Mollenhauer, H.H., Koput, C.: Chromoplast development in daffodil. J. Microscopie7, 1045–1050 (1968)
Mudd, J.B., Garcia, R.E.: Biosynthesis of glycolipids. In: Recent Advances in the Chemistry and Biochemistry of Plant Lipids (T. Galliard, E.I. Mercer, eds.), pp. 161–201. London-New York-San Francisco: Academic Press 1975
Ongun, A., Mudd, J.B.: The biosynthesis of steryl glucosides in plants. Plant Physiol.45, 255–262 (1970)
Péaud-Lenoel, C., Axelos, M.: Uridine diphosphate glucose: stérol transglucosylase. Purification et activité de préparations particuliaires et solubles extraites du blé. C.R. Acad. Sc. Paris, Ser. D273, 1057–1060 (1971)
Poincelot, R.P.: Isolation and lipid composition of spinach chloroplast envelope membranes. Arch. Biochem.159, 134–142 (1973)
Rebeiz, C.A., Castelfranco, P.A.: Protochlorophyll and chlorophyll biosynthesis in cell-free systems from higher plants. Ann. Rev. Plant Physiol.24, 129–172 (1973)
Roughan, P.G.: Phosphatidyl choline donor of 18-carbon unsaturated fatty acids for glycerolipid biosynthesis. Lipids10, 609–614 (1975)
Shine, W.E., Mancha, M., Stumpf, P.K.: Fat metabolism in higher plants. Differential incorporation of acyl-coenzymes A and acyl-acyl carrier proteins into plant microsomal lipids. Arch. Biochem.173, 472–479 (1976)
Thomas, G., Threlfall, D.R.: Synthesis of polyprenyltoluquinols from homogentisate and polyprenyl pyrophosphates in particulate fractions ofEuglena and sugar beet. Biochem. J.142, 437–440 (1974)
Van Hummel, H.C., Hulsebos, T.J.M., Wintermans, J.F.G.M.: Biosynthesis of galactosyl diglycerides by non-green fractions from chloroplasts. Biochim. Biophys. Acta380, 219–226 (1975)
Williams, J.P., Watson, G.R., Leung, S.P.K.: Galactolipid synthesis inVicia faba leaves. II. Formation and desaturation of long chain fatty acids in phosphatidyl choline, phosphatidyl glycerol and the galactolipids. Plant Physiol.57, 179–184 (1976)
Wojciechowski, Z.A., Zimowski, J.: Acyl composition and biosynthesis of acylated steryl glucosides inCalendula officinalis. Biochim. Biophys. Acta398, 111–117 (1975)
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Liedvogel, B., Kleinig, H. Lipid metabolism in chromoplast membranes from the daffodil: Glycosylation and acylation. Planta 133, 249–253 (1977). https://doi.org/10.1007/BF00380685
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DOI: https://doi.org/10.1007/BF00380685