Abstract
A cytokinin was isolated from the culture medium of callus cells of the moss hybridFunaria hygrometrica (L.) Sibth xPhyscomitrium piriforme Brid. The purification procedure included ethyl-acetate extraction, silver-salt precipitation, crystallization as picrate, and ion exchange chromatography. The structure of the cytokinin was confirmed as N6−(Δ2-isopentenyl)adenine by means of gas chromatography and mass spectrometry. The concentration of the compound in the culture medium was determined at ca. 10-6 M.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- 2iP:
-
N6−(Δ2-isopentenyl) adenine
References
Bauer, L.: Über die Kalluswuchsform des Laubmoossporophyten. Naturwissenschaften48, 507–508 (1961)
Bauer, L.: On the physiology of sporogonium differentiation in mosses. J. Linn. Soc. (Bot.)58, 333–351 (1963)
Bauer, L.: Isolierung und Testung einer kinetinartigen Substanz aus Kalluszellen von Laubmoossporophyten. Z. Pflanzenphysiol.54, 241–253 (1966)
Beutelmann, P.: Untersuchungen zur Biosynthese eines Cytokinins in Calluszellen von Laubmoossporophyten. Planta112, 181–189 (1973)
Dyson, W.H., Hall, R.H.: N6−(Δ2-isopentenyl) adenosine: its occurrence as a free nucleoside in an autonomous strain of tobacco tissue. Plant Physiol.50, 616–621 (1972)
Helgeson, J.P., Leonard, N.J.: Cytokinins: identification of compounds isolated fromCorynebacterium fascians. Proc. nat. Acad. Sci. (Wash.)56, 60–63 (1966)
Klämbt, D.: Thies G., Skoog, F.: Isolation of cytokinins fromCorynebacterium facians. Proc. nat. Acad. Sci. (Wash.)56, 52–63 (1966)
Klämbt, D.: Cytokinine ausHelianthus annuus. Planta82, 170–178 (1968)
Letham, D.S.: Isolation of a kinin (zeatin) from plum fruitlets and other tissues. In: Regulateurs naturels de la croissance végétales, Coll. nat. Rech. Sci.123, 109–117 (1964)
Letham, D.S., Shannon, J.S., McDonald, I.R.C.: Regulators of cell division in plant tissues. III. The identity of zeatin. Tetrahedron23, 479–486 (1967)
Linstedt, D., Reinert, J.: Occurrence and properties of a cytokinin in tissue cultures ofDaucus carota. Naturwissenschaften62, 238 (1975)
Miller, C.O.: Evidence for the natural occurrence of zeatin and derivatives: compounds from maize, which promote cell division. Proc. nat. Acad. Sci. (Wash.)54, 1052–1058 (1965)
Shindy, W.W., Smith, O.E.: Identification of plant hormones in cotton ovules. Plant Physiol.55, 550–554 (1975)
Upper, C.D., Helgeson, J.P., Kemp, J.D., Schmidt, C.J.: 6-(3-methyl-2-buteny)aminopurine fromAgrobacterium tumefaciens. Plant Physiol.45, 543–547 (1970)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Beutelmann, P., Bauer, L. Purification and identification of a cytokinin from moss callus cells. Planta 133, 215–217 (1977). https://doi.org/10.1007/BF00380679
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00380679