Abstract
Enzymic activities have been measured in cell-free extracts from nitrogen-starved cultures ofAnkistrodesmus braunii. During ten hours of nitrogenstarvation the activities of the enzymes nitrite reductase (E.C.1.6.6.4), glutamic dehydrogenase (E.C.1.4.1.4), glutamine synthetase (E.C.6.3.1.2) and urea amidolyase (E.C.3.5.1.5) were derepressed while the activities of the enzymes malate dehydrogenase (E.C.1.1.1.37) and hexokinase (E.C.2.7.1.1) remained more or less unchanged. In contrast, the photosynthetic capacity of the nitrogen-starved cultures declined rapidly and accompanying this decline were losses in the activities of ribulose diphosphate carboxylase (E.C.4.1.1.39) and triose phosphate-NADP-dehydrogenase (E.C.1.2.1.13).
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References
Aach, H.G.: Über Wachstum und Zusammensetzung vonChlorella pyrenoidosa bei unterschiedlichen Lichtstärken und Nitratmengen. Arch. Mikrobiol.17, 231–246 (1952)
Adams, A.A.: The regulation of nitrogen assimilation inChlorella. Ph.D. Thesis, University of Wales (1971)
Amy, N.K., Garrett, R.H.: Purification and characterisation of the nitrate reductase from the diatomThalassiosira pseudonana. Plant Physiol.54, 629–637 (1974)
Bongers, L.H.J.: Aspects of nitrogen assimilation by cultures of algae. Meded. Land. Hoogesch. Wageningen56, 1–52 (1956)
Fogg, G.E.: Nitrogen nutrition and metabolic patterns in algae. Symp. Soc. Exp. Biol.13, 106–125 (1959)
Fogg, G.E.: Algal culture and phytoplankton ecology. Milwaukee and London: University of Wisconsin Press, Madison 1966
Frieden, C.: L-Glutamate dehydrogenase. In: The enzymes. 7, 2nd ed., 3–24, Boyer, P.D., Lardy, H., Myrbak, K., eds. New York: Academic Press 1963
Halvorson, H.: Intracellular protein and nucleic acid turnover in resting yeast cells. Biochem. Biophys. Acta27, 255–268 (1958)
Hartree, E.F.: A modification of the Lowry method that gives a linear photometric response. Analyt. Biochem.48, 422–427 (1972)
Hattori, A.: Studies on the metabolism of urea and other nitrogenous compounds inChlorella ellipsoidea. I. Assimilation of urea and other nitrogenous compounds by nitrogen-starved cells. J. Biochem.44, 253–273 (157)
Healey, F.P.: Inorganic nutrient uptake and deficiency in algae. Critic. rev. microbiol.3, 69–113 (1973)
Hewitt, B.R.: Spectrophotometric determination of protein in alkaline solution. Nature (Lond.)182, 246 (1958)
Hodson, R.C., Williams, J.K., Davidson, W.R.: Metabolic control of urea catabolism inChlamydomonas reinhardi andChlorella pyrenoidosa. J. Bacteriol.121, 1022–1035 (1975)
Lea, P.J., Miflin, B.J.: The occurrence of glutamate synthase in algae. Biochem. Biophys. Res. Commun.64, 856–862 (1975)
Leftley, J.W., Syrett, P.J.: Urease and ATP: urea amidolyase activity in unicellular algae. J. Gen. Microbiol.77, 109–115 (1973)
Loomis, W.D.: Amide metabolism in higher plants III. Distribution of glutamyl transferase and glutamine synthetase activity. Plant. Physiol.34, 541–546 (1959)
Morris, Syrett, P.J.: The effect of nitrogen-starvation on the activity of nitrate reductase and other enzymes inChlorella. J. Gen. Microbiol.38, 21–28 (1965)
Morton, A.G., Dickerson, A.G.F., England, D.J.F.: Changes in enzyme activity of fungi during nitrogen-starvation. J. exp. Bot.11, 116–128 (1960)
North, B.B., Stephens, G.C.: Uptake and assimilation of amino acids byPlatymonas. II. Increased uptake in nitrogen-deficient cells. Biol. Bull.140, 242–254 (1971)
Öesterheld, H.: Das Verhalten von Nitrat Reductase, Nitrit Reductase, Hydrogenase und anderen Enzymen vonAnkistrodesmus braunii bei Stickstoffmangel. Arch. Mikrobiol.79, 25–43 (1971)
Oshia, Y., Hase, H.: Changes in ribulose-1,5 diphosphate carboxylase levels during the process of degeneration and regeneration of chloroplass inChlorella protothecoides. Plant Cell Physiol.13, 955–963 (1972)
Pateman, J.A.: Regulation of the synthesis of glutamate dehydrogenase and glutamine synthetase in micro-organisms. Biochem. J.115, 769–775 (1970)
Rigano, C., Violante, U.: Effect of nitrate, ammonia and nitrogen starvation on the regulation of nitrate reductase inCyanidium caldarium. Arch. Mikrobiol.90, 27–33 (1973)
Shapiro, B.M., Stadtman, E.R.: The regulation of glutamine synthesis in microorganisms. Ann. Rev. Microbiol.24, 501–524 (1970)
Snell, F.D., Snell, C.T.: Colormetric methods of analysis. Vol. II, 3rd ed., pp. 785–807. New York: Van Nostrand 1949
Spoehr, H.A., Milner, H.W.: The chemical composition ofChlorella; effect of environmental conditions. Plant Physiol.24, 120–149 (1949)
Syrett, P.J.: The assimilation of ammonia by nitrogen-starved cells ofChlorella vulgaris. I. The correlation of assimilation with respiration. Ann. Bot. (Lond.)17, 21–36 (1953)
Syrett, P.J.: The assimilation of ammonia by nitrogen-starved cells ofChlorella vulgaris. II. The assimilation of large quantities of nitrogen. Physiol. Plant.9, 19–27 (1956)
Syrett, P.J., Hipkin, C.R.: The appearance of nitrate reductase activity in nitrogen-starved cells ofAnkistrodesmus braunii, Planta (Berl.)111, 57–64 (1973)
Syrett, P.J., Leftley, J.W.: Nitrate and urea assimilation in algae. In: Perspectives in experimental biology, Vol. 2, Botany, pp. 221–234, Sunderland, N., Ed., Oxford: Pergamon Press 1976
Thomas, W.H., Dodson, A.N.: On nitrogen-deficiency in tropical Pacific oceanic phytoplankton. II. Photosynthesis and cellular characteristics of a chemostat grown diatom. Limnol. Oceanog.17, 515–523 (1972)
Thomas, W.H., Krauss, R.W.: Nitrogen metabolism inScendeesmus as affected by environmental changes. Plant Physiol.30, 113–122 (1955)
Thompson, J.F., Muenster, A.M.E.: Separation of theChlorella ATP: urea amido lyase into two components. Biochem. Biophys. Res. Commun.43, 1049–1055 (1971)
Thompson, J.F., Muenster, A.M.E.: ATP-dependent urease: characterization of and control inChlorella; the search for it in higher plants. Bull. Roy. Soc. New Zealand12, 91–97 (1974)
Zumft, W.G., Spiller, H., Yeboah-Smith, I.: Eisengehalt und Elektrondonator-Spezifität der Nitratreductase vonAnkistrodesmus. Planta (Berl.)102, 228–236 (1971)
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Hipkin, C.R., Syrett, P.J. Some effects of nitrogen-starvation on nitrogen and carbohydrate metabolism inAnkistrodesmus braunii . Planta 133, 209–214 (1977). https://doi.org/10.1007/BF00380678
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DOI: https://doi.org/10.1007/BF00380678