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Beziehungen zwischen Carboanhydraseaktivität und Aufnahme von HCO3 − und Cl− bei der Photosynthese von Scenedesmus obliquus

Relations between carbonic anhydrase activity and uptake of HCO3 - and Cl- in photosynthesis by Scenedesmus obliquus

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Summary

Synchronized, young cells of Scenedesmus obliquus when adapted to air plus 1.5% CO2 have only about 1/20 of the carbonic anhydrase activity of air-adapted cells. At pH 9.2 (where HCO3 - is the prevailing form of inorganic carbon) such cultures do not evolve much O2 at 50·103 lux, in contrast to air-adapted cells. In contrast, at pH 5.8 (where CO2 prevails) there is not much difference in O2 evolution rate between cultures adapted to different CO2-levels. It is concluded that carbonic anhydrase activity is necessary for the utilisation of HCO3 - but not of CO2 in photosynthesis by Scenedesmus.

Air-adapted cells take up about 0.3 μmoles Cl-/g FW from 0.1 mM KCl solution (pH 5.8) within the first minute of illumination. The same amount is released when the light is switched off. The light induced Cl--uptake is inhibited by addition of HCO3 - or high pH, and may be interpreted as an alternative uptake of Cl- instead of HCO3 -.

As cells adapted to air plus 1.5% CO2 do not show this light induced Cl--uptake and as the inhibitor of carbonic anhydrase, diamox, affects Cl--uptake of air-adapted cells, it is suggested that carbonic anhydrase may be involved in the uptake of Cl- and HCO3 -.

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  1. Fachbereich Biologie, Botanik, Technische Hochschule Darmstadt, Schnittspahnstr. 3-5, D-6100, Darmstadt, Federal Republic of Germany

    Günter R. Findenegg

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  1. Günter R. Findenegg
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Findenegg, G.R. Beziehungen zwischen Carboanhydraseaktivität und Aufnahme von HCO3 − und Cl− bei der Photosynthese von Scenedesmus obliquus . Planta 116, 123–131 (1974). https://doi.org/10.1007/BF00380647

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  • DOI: https://doi.org/10.1007/BF00380647

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