Summary
[3H]Gibberellin A20 ([3H]GA20), a native gibberellin of this plant, was injected into mature leaves of Bryophyllum daigremontianum (Hamet et Perr.) Berg. under long- and short-day conditions. It was converted, in order of decreasing yields, to GA29, 3-epi-GA1 (pseudo GA1), C/D-ring-rearranged GA20, and two minor, unidentified metabolites. Identifications were made by gas-liquid chromatography with radioactive monitoring using three different phases. Metabolism to 3-epi-GA1 was greater under short days, particularly in the treated leaf pair, although the absolute amount of GA29 was greater than that of 3-epi-GA1 under both photoperiods. The levels of radioactive metabolites in the shoots above the treated leaf pair gradually increased over a 51-day period, GA29 reaching 5 times the contten of 3-epi-GA1.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- GA:
-
gibberellin
- GC-RC:
-
gas-liquid chromatography with radioactive monitoring
- GC-MS:
-
combined gas chromatography-mass spectrometry
- TLC:
-
thin-layer chromatography
- TMSiMe:
-
trimethylsilyl ether methylester
- LD:
-
long day(s)
- SD:
-
short day(s)
- f.c.:
-
foot candles
References
Cavell, B. D., MacMillan, J., Pryce, R. J., Sheppard, A. C.: Thin-layer and gas-liquid chromatography of the gibberellins: direct identification of the gibberellins in a crude plant extract by gas-liquid chromatography. Phytochemistry 6, 867–874 (1967)
Cross, B. E., Grove, J. F., Morrison, A.: Gibberellic acid. Part XVIII. Some rearrangements of ring A. J. chem. Soc. 1961, 2498–2515
Durley, R. C., Crozier, A., Pharis, R. P., McLaughlin, G. E.: Chromatography of 33 gibberellins on a gradient eluted silica gel partition column. Phytochemistry 11, 3029–3033 (1972)
Durly, R. C., Railton, I. D., Paris, R. P.: Interconversion of gibberellin A5 to gibberellin A3 in seedlings of dwarf Pisum sativum. Phytochemistry 12, 1609–1612 (1973)
Frydman, V. M., MacMillan, J.: Identification of gibberellins A20 and A29 in seed of Pisum sativum cv. Progress No. 9 by combined gas chromatography-mass spectrometry. Planta (Berl.) 115, 11–15 (1973)
Frydman, V. M., Gaskin, P., MacMillan, J.: Qualitative and quantitative analyses of gibberellins throughout seed maturation in Pisum sativum cv. Progress No. 9. Planta (Berl.) 118, 123–132 (1974)
Gaskin, P., MacMillan, J., Zeevaart, J. A. D.: Identification of gibberellin A20, abscisic acid, and phaseic acid from flowering Bryophyllum daigremontianum by combined gas chromatography-mass spectrometry. Planta (Berl.) 111, 347–352 (1973)
Glenn, J. L., Kuo, C. C., Durley, R. C., Pharis, R. P.: Use of insoluble polyvinylpyrrolidone for purification of plant extracts and chromatography of plant hormones. Phytochemistry 11, 345–351 (1971)
Hashimoto, T., Tamura, S., Mori, K., Matsui, M.: Inhibitive and promotive effects of desoxygibberellin C and its methyl ester on plant growth. Plant Physiol. 42, 886–888 (1967)
Kato, J., Katsumi, M.: Pseudogibberellin A1 as an inhibitor of the GA3-induced growth of rice seedlings. Planta (Berl.) 74, 194–196 (1967)
Mori, K., Matsui, M., Sumiki, Y.: Biochemical studies on bakanae fungus. Part 65. Synthesis of substances related to gibberellins. IX. Relactonization of degradation products of gibberellic acid. Agr. Biol. Chem. 27, 530–536 (1963)
Murofushi, N., Durley, R. C., Pharis, R. P.: Preparation of radioactive gibberellins A20, A5 and A8. Agr. Biol. Chem. 38, 475–476 (1974)
Railton, I. D.: Studies on gibberellins in shoots of light-grown peas II. The metabolism of tritiated gibberellin A9 and gibberellin A20 by light- and dark-grown shoots of dwarf Pisum sativum var. Meteor. Plant Sci. Lett. 3, 207–212 (1974)
Railton, I. D., Murofushi, N., Durley, R. C., Pharis, R. P.: Interconversion of gibberellin A20 to gibberellin A29 by etiolated seedlings and germinating seeds of dwarf Pisum sativum. Phytochemistry 13, 793–796 (1974)
Reeve, D. R., Crozier, A.: An assessment of gibberellin structure-activity relationships. J. exp. Bot. 25, 431–445 (1974)
Stolp, C. F., Nadeau, R., Rappaport, L.: Effect of abscisic acid on uptake and metabolism of [3H]gibberellin A1 and [3H]pseudogibberellin A1 by barley half-seeds. Plant Physiol. 52, 546–548 (1973)
Takahashi, N., Kitamura, H., Kawarada, A., Seta, Y., Takai, M., Tamura, S., Sumiki, Y.: Biochemical studies on “bakanae” fungus. Part XXXIV. Isolation of gibberellins and their properties. Bull. Agr. Chem. Soc. Japan 19, 267–277 (1955)
Yokota, T., Murofushi, N., Takahashi, N., Tamura, S.: Gibberellins in immature seeds of Pharbitis nil Part III. Isolation and structure of gibberellin glucosides. Agr. Biol. Chem. 35, 583–595 (1971a)
Yokota, T., Murofushi, N., Takahashi, N.: Biological activities of gibberellins and their glucosides in Pharbitis nil. Phytochemistry 10, 2943–2949 (1971b)
Zeevaart, J. A. D.: Gibberellin-like substances in Bryophyllum daigremontianum and the distribution and persistence of applied gibberellin A3. Planta (Berl.) 86, 124–133 (1969a)
Zeevaart, J. A. D.: Changes in the gibberellin content of Bryophyllum daigremontianum in connection with floral induction. Neth. J. agric. Sci. 17, 215–220 (1969b)
Zeevaart, J. A. D.: Gibberellin A20 content of Bryophyllum daigremontianum under different photoperiodic conditions as determined by gas-liquid chromatography. Planta (Berl.) 114, 285–288 (1973)
Author information
Authors and Affiliations
Additional information
J. A. D. Z. was supported by the United States Atomic Energy Commission under Contract AT(11-1)-1338; R. P. P. by the National Research Council of Canada Grant No. A-2585.
Rights and permissions
About this article
Cite this article
Durley, R.C., Pharis, R.P. & Zeevaart, J.A.D. Metabolism of [3H]Gibberellin A20 by plants of Bryophyllum daigremontianum under long- and short-day conditions. Planta 126, 139–149 (1975). https://doi.org/10.1007/BF00380617
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00380617