Summary
The effect of various heme compounds on growth and maturation of Hymenolepis microstoma in vitro has been determined. At 4 days p.i., worms were collected and cultured in vitro for 6 days in a medium containing various concentrations of haemoglobin, hemin, and bilirubin.
Addition of haemoglobin to a medium resulted in significant increases in length, wet weight, number of immature and mature proglottids as compared to the control medium. Supplements of hemin did not seem to affect worm length; however, they caused a significant weight increase, and the numbers of immature and mature proglottids were also more than those in control medium. Bilirubin supplements failed to show any effect on growth and maturation of H. microstoma, and the experimental and control worms were found similar in all respects.
In the light of the success with haemoglobin and hemin as growth promoting substances, the role of blood ingredients was also considered. For strobilization and maturation of H. microstoma in vitro, the presence of some kind of heme protein seems essential.
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References
Berntzen, A. K.: The in vitro cultivation of tapeworms. I. Growth of Hymenolepis diminuta (Cestoda: Cyclophyllidea). J. Parasit. 47, 351–355 (1961)
Crofton, D. H.: Nematodes. London: Hutchinson University Library 1966
Evans, W. S.: In vitro cultivation of Hymenolepis microstoma from cysticercoid to egg producing adult. Canad. J. Zool. 48, 1135–1137 (1970)
Heath, D. D.: An improved technique for the in vitro cultivation of taeniid larvae. Int. J. Parasit. 3, 481–484 (1973)
Hieb, W. F., Stokstad, E. L. R., Rothstein, M.: Heme requirement for reproduction of a free-living nematode. Science 168, 143–144 (1970)
Khan, Z. I., De Rycke, P. H.: The in vitro cultivation of Hymenolepis microstoma with particular reference to the role of serum for strobilization and gametogenesis. Biol. Jb. Dodonaea 43, 151–172 (1975)
Lascelles, J.: Tetrapyrrole synthesis in microorganisms. In: Gunsalus and R. Stanier (eds.), The bacteria, Vol. III, p. 335. New York: Academic Press 1962
Schiller, E. L.: A simplified method for the in vitro cultivation of the rat tapeworm, Hymenolepis diminuta. J. Parasit. 51, 516–518 (1965)
Seidel, J. S.: Hemin as requirement in the development in vitro of Hymenolepis microstoma (Cestoda: Cyclophyllidea) J. Parasit. 57, 566–570 (1971)
Simpson, L.: The Leishmania-leptomonade transformation of Leishmania donovani: nutritional requirements, respiration changes and antigenic changes. J. Protozool. 15, 201–217 (1968)
Vanfleteren, J. R.: Nematode growth factor. Nature (London.) 248, 225–227 (1974)
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The senior author gratefully acknowledges a fellowship from the State University of Gent
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Khan, Z.I., De Rycke, P.H. Studies on Hymenolepis microstoma in vitro. Z. Parasitenk 49, 253–261 (1976). https://doi.org/10.1007/BF00380595
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DOI: https://doi.org/10.1007/BF00380595