Summary
The excretory canals of Ascaridia galli (Nematoda) and the protonephridial ducts of Cotylophoron cotylophorum (Trematoda) and Raillietina cesticillus (Cestoda) have been studied with regard to the histochemical localization of lipids, carbohydrates and hydrolytic enzymes. Distinct excretory organs are absent in the acanthocephalan Centrorhynchus corvi. Triglycerides, phospholipids and lipoproteins are seen in association with the wall of excretory canals of A. galli and R. cesticillus, and phospholipids and lipoproteins at the corresponding site in C. cotylophorum. The physiological significance of lipids in association with excretion of substances has been discussed. Low molecular weight glycogen is present in the lumen of excretory canal of A. galli but not in other worms.
The common feature of the excretory canals is the presence of enzyme activities of nonspecific alkaline phosphatase and Mg2+-dependent ATPase. Activity of acid phosphatase is seen only in the excretory canals of A. galli. Glucose-6-phosphatase is present in A. galli and C. cotylophorum and absent in R. cesticillus. Weak reaction of 5′-nucleotidase is present in the excretory canals of helminth species studied here. The role of these enzymes in transportation of substances across the wall of excretory canals and also in ionic regulation has been discussed in detail.
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References
Bird, A.F.: The structure of nematodes. New York and London: Academic Press 1971
Branch, S.I.: Moniliformis dubius and Macracanthorhynchus hirudinaceus: Na, K, Ca and Mg content, and Na and K active transport. Exp. Parasit. 27, 33–43 (1970a)
Branch, S.I.: Moniliformis dubius: osmotic responses. Exp. Parasit. 27, 44–52 (1970b)
Brand, T. von: Biochemistry of parasites. New York and London: Academic Press 1973
Burren, C.H., Ehrlich, I., Johnson, P.: Excretion of lipids by the liver fluke (Fasciola hepatica L.). Lipids 2, 353–356 (1967)
Burstone, M.S.: Histochemical demonstration of phosphatases in frozen sections with naphthol AS-MX phosphates. J. Histochem. Cytochem. 9, 146–153 (1961)
Erasmus, D.A.: The phosphatase systems of cestodes. Part 2. Studies on Cysticercus tenuicollis and Moniezia expansa (adult). Parasitology 47, 81–91 (1957)
Erasmus, D.A.: Ultrastructural observations on the reserve bladder system of Cyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea) with special reference to lipid excretion. J. Parasit. 53, 525–536 (1967)
Gomori, G.: Microscopic histochemistry. Chicago: University of Chicago Press 1952
Gupta, A.N., Guraya, S.S., Sharma, P.N.: Histochemical observations on the excretory system of digenetic trematodes. Acta morph. neerl.-scand. 12, 231–242 (1974)
Halton, D.W.: Studies on phosphatase activity in Trematoda. J. Parasit. 53, 46–54 (1967)
Hedrick, R.M.: Comparative histochemical studies on cestodes. II. The distribution of fat substances in Hymenolepis diminuta and Raillietina cesticillus. J. Parasit. 44, 75–85 (1958)
Howells, R.E.: Observations on the nephridial system of cestode Moniezia expansa (Rud., 1805). Parasitology 59, 449–459 (1969)
Kaplow, L.S., Burstone, M.S.: Cytochemical demonstration of acid phosphatase in hematopoietic cells in health and in various haematological disorders using azo dye techniques. J. Histochem. Cytochem. 12, 805–811 (1964)
Kirschner, L.B.: Comparative physiology: Invertebrate excretory organs. A. Rev. Physiol. 29, 169–196 (1967)
Lee, D. L., Tatchell, R.J.: Studies on the tapeworm Anoplocephala perfoliata. Parasitology 54, 467–479 (1964)
Martin, A.W., Bils, R.F.: Trematode excretory concretions: Formation and fine structure. J. Parasit. 50, 337–344 (1964)
Moss, G.D.: The excretory metabolism of the endoparasitic digenean Fasciola hepatica and its relationship to its respiratory metabolism. Parasitology 60, 1–19 (1970)
Nicholas, W.L.: The biology of the acanthocephala. Advanc. Parasit. 5, 205–246 (1967)
Pantelouris, E.M., Threadgold, L.T.: The excretory system of adult Fasciola hepatica. Cellule 64, 63–67 (1963)
Pappas, P.W., Read, C.P.: Membrane transport in helminth parasites. A review. Exp. Parasit. 37, 469–530 (1975)
Parshad, V.R. Guraya, S.S.: Comparative histochemical observations on the lipids in the immature and mature stages of Cotylophoron cotylophorum (Paramphistomatide: Digenea). J. Helminth. 50, 11–15 (1976)
Pearse, A.G.E.: Histochemistry: theoretical and applied. London: Churchill 1968
Read, C.P.: Nutrition of intestinal helminths. In: Biology of parasites, E.J.L. Soulsby, ed. New York: Academic Press 1966
Threadgold, L.T.: Electron microscope studies of Fasciola hepatica. VI. The ultrastructural localization of phosphatases. Exp. Parasit. 23, 264–276 (1968)
Tormey, J. Mc. D: Significance of the histochemical demonstration of ATPase in epithelia noted for active transport. Nature 210, 820–822 (1966)
Webster, L.A.: Absorption of glucose, lactate and urea from the protonephridial canals of Hymenolepis diminuta. Comp. Biochem. Physiol. 41, 861–868 (1972)
Webster, L.A., Wilson, R.A.: The chemical composition of protonephridial canal fluid from the cestode Hymenolepis diminuta. Comp. Biochem. Physiol. 35, 201–209 (1970)
Wilson, R.A., Webster, L.A.: Protonephridia. Biol. Rev. 49, 127–160 (1974).
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Parshad, V.R., Guraya, S.S. Comparative histochemical observations on the excretory system of helminth parasites. Z. F. Parasitenkunde 52, 81–89 (1977). https://doi.org/10.1007/BF00380561
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DOI: https://doi.org/10.1007/BF00380561