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Enzymaktivitäten im Siebröhrensaft von Robinia pseudoacacia L. und anderer Baumarten

Enzyme activities in the sieve tube sap of Robinia pseudoacacia L. and of other tree species

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Summary

  1. 1.

    Electron microscopic studies of the sieve tube sap obtained from the secondary phloem of Robinia pseudoacacia by the method of Hartig (1860) showed the presence of well developed mitochondria in addition to membrane fragments.

  2. 2.

    In this sieve tube sap the following enzymes could be detected qualitatively: UTP-glucose-1-phosphate-uridyl transferase, UDPG-fructose glucosyl transferase, glucose-6-phosphate dehydrogenase, hexokinase (for glucose and fructose), phosphohexose isomerase, phosphofructokinase, and UDPG-pyrophosphatase.

  3. 3.

    The following enzymes were determined quantitatively: phosphorylase, amylase, aldolase, triosephosphate isomerase, NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase, phosphoglyceromutase, enolase, pyruvate kinase, pyruvate decarboxylase, alcohol dehydrogenase, isocitrate dehydrogenase, fumarase, malate dehydrogenase, glutamate-pyruvate transaminase, glutamate dehydrogenase, glutamate-oxalacetate transaminase, and anorganic pyrophosphatase.

  4. 4.

    The following enzymes could not be detected: UDGP dehydrogenase, UDPG-fructose-6-phosphate-glucosyltransferase, invertase, phosphoglucomutase, lactate dehydrogenase, and citrate synthase.

  5. 5.

    The enzyme pattern in the sieve tube saps of Tilia platyphyllos, Carpinus betulus, Fraxinus americana, Quercus borealis maxima, and Salix viminalis is qualitatively similar to that of Robinia, but shows quantitative differences (as far as analyzed).

  6. 6.

    The meaning of the results for the metabolism and function of the sieve tubes in situ is discussed.

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Literatur

  • Atkinson, D. E.: Enzyme modulation and metabolic regulation. Vortrag auf dem XI. Internat. Bot. Congr., Seattle 1969.

  • Bianchetti, R., Marré, E.: Sul trasporto dei glucidi nelle piante susperori, I. Aspetti biochimici, Giorn. Bot. Ital. 69, 299–311 (1962).

    Google Scholar 

  • —, Sartirana, M. L.: AMP-sensitive fructose diphosphatase in higher plants: Effects of carbon sources on the level of the enzyme. Biochem. biophys. Res. Commun. 27, 278–283 (1967).

    Google Scholar 

  • Boehringer, C.F.: Arbeitsvorschriften zu Testkombinationen, Mannheim, 1964. Biochemica Information, Mannheim, 1961.

  • Cardini, C. E., Leloir, L. F., Chiriboga, J.: The biosynthesis of sucrose. J. biol. Chem. 214, 149–155 (1955).

    Google Scholar 

  • Evans, N. T. S., Ebert, M., Moonby, J.: A model for translocation of photosynthate in the soybean. J. exp. Bot. 14, 221–231 (1963).

    Google Scholar 

  • Fekete, M. A. R. de: Interaction of amyloplasts in Vicia faba. Arch. Biochem 116, 368–374 (1966).

    Google Scholar 

  • —, Ziegler, H., Wolf, R.: Enzyme des Kohlenhydratstoffwechsels in Nektarien. Planta (Berlin) 75, 125–138 (1967).

    Google Scholar 

  • Gardner, D. C., Peel, A. J.: ATP in sieve tube sap from willow. Nature (London) 222, 774 (1969).

    Google Scholar 

  • Green, A. A., Hughes, W. L.: Protein fractionation on the basis of solubility in aqueous solutions of salts and organic solvents. In: Methods in enzymology (S. P. Colowick and N. O. Kaplan, eds.) vol. 1, p. 67–90. New York: Academic Press 1955.

    Google Scholar 

  • Hartig, Th.: Beiträge zur physiologischen Forstbotanik. Allg. Forst-u. Jagd-Ztg. 36, 257–261 (1860).

    Google Scholar 

  • Hatch, M. D., Glasziou, K. T.: Direct evidence for translocation of sucrose in sugarcane leaves and trees. Plant Physiol. 39, 180–184 (1964).

    Google Scholar 

  • Heppel, L. A.: Inorganic pyrophosphatase from yeast. In: Methods of enzymology (S. P. Colowick and N. O. Kaplan, eds.) vol. 2, p. 570–576. New-York: Academic Press 1955

    Google Scholar 

  • Hewitt, E. J., Nicholas, D. J. D.: Cations and anions. Inhibitions and interactions in enzyzyme activity. In: Metabolic Inhibitors (R. M. Hochster and J. H. Quastel, eds.), vol. 2., p. 311–436. New York-London: Academic Press 1963.

    Google Scholar 

  • Jauch, R., Riepertinger, Ch., Lynen, F.: Reinigung und Eigenschaften der Phosphofructokinase aus Hefe. Hoppe-Seylers Z. physiol. Chem. 351, 74–80 (1970).

    Google Scholar 

  • Kluge, M.: Untersuchungen über die chemische Zusammensetzung von Siebröhrensäften. Diss., Techn. Hochschule Darmstadt 1964.

  • —, Becker, D., Ziegler, H.: Untersuchungen über ATP und andere organische Phosphorverbindungen im Siebröhrensaft von Yucca flaccida und Salix triandra. Planta (Berlin) 91, 68–79 (1970).

    Google Scholar 

  • —, Ziegler, H.: Der ATP-Gehalt der Siebröhrensäfte von Laubbäumen. Planta (Berlin) 61, 167–177 (1964).

    Google Scholar 

  • Krisman, C. R.: A method for the colorimetric estimation of glycogen with iodine. Analyt. Biochem. 4, 17–23 (1962).

    Google Scholar 

  • Kursanov, A. L.: The transport of organic substances in plants. Endeavour 20, 19–25 (1961).

    Google Scholar 

  • —, Pavlinova, O. A., Afanasieva, T. P.: The glycolytic enzymes in the conducting tissues of sugarbeet. Arch. Biochem. 83, 239–247 (1959).

    Google Scholar 

  • —, Sokolova, S. V., Turkina, M. V.: Hexokinase of the conducting bundles of sugar beet plants. Localisation, properties and its possible relation to hexose transport through plant membrans. Fiziol. Rastenij 16, 786–794 (1969) (russ, mit engl., Zus. fass.).

    Google Scholar 

  • Layne, E.: Spectrophotometric and turbidimetric methods for measuring proteins. In: Methods of enzymology (S. P. Colowick and N. O. Kaplan, eds.) vol. 3, p. 447–454. New York: Academic Press 1957.

    Google Scholar 

  • Leloir, L. F., Cardin, C. E.: Characterization of phosphorus compounds by acid lability. In: Methods of enzymology (S. P. Colowick and N. O. Kaplan, eds.), vol. 3, p. 840–850. New York: Academic Press 1957.

    Google Scholar 

  • Linskens, H. F.: Papierchromatographie in der Botanik 2. Aufl. Berlin-Göttingen-Heidelberg: Springer 1959.

    Google Scholar 

  • Mahler, H. R., Cordes, E. H.: Biological chemistry. Harper Internat. Ed. 1967.

  • Neumann, St., Wollgiehn, R.: Über die Bezichungen der RNS- und Proteinsynthese zum Zellkern in Siebzellen von Vicia faba. Z. Naturforsch. 196, 1066–1071 (1964).

    Google Scholar 

  • Ochoa, S.: Cristalline condensing enzyme from pig heart. In: Methods of enzymology (S. P. Colowick and N. O. Kaplan, eds.), vol. 1. p. 685–694. New York: Academic Press 1955.

    Google Scholar 

  • Paladini, A. C., Leloir, L. F.: Studies on uridine-diphosphate-glucose. Biochem. J. 51, 426–430 (1952).

    Google Scholar 

  • Racker, E.: Spectrophotometric measurements on the enzymatic formation of fumaric and cis-aconitic acids. Biochim. biophys. Acta (Amst.) 4, 211–214 (1950).

    Google Scholar 

  • Reimer, L.: Elektronenmikroskopische Untersuchungs-und Präparationsmethoden. Berlin-Heidelberg-New York: Springer 1967.

    Google Scholar 

  • Schuhmacher, W.: Der Stofftransport in der Pflanze. In: Handbuch der Pflanzenphysiologie (W. Ruhland, Ed.), Bd. 13. Berlin-Heidelberg-New York: Springer 1967

    Google Scholar 

  • Strauss, J.: Invertase in cell walls of plant tissue cultures. Plant Physiol 37, 342–348 (1962).

    Google Scholar 

  • Trevelyan, W. E., Procter, D. P., Harrison, J. S.: Detection of sugars on paper chromatograms. Nature (Lond.) 166, 444 (1950).

    Google Scholar 

  • Turkina, M. W., Dubinina, I. M.: Einige Besonderheiten des Atmungssystems der Leitbündel. Dokl. Akad. Nauk, SSSR 95, 199–202 (1954) [russ.].

    Google Scholar 

  • Ulbrich, M.: Lokalisation und Eigenschaften von Phosphatase in Nektarien und Phloemgewebe. Diplomarbeit Techn. Hochschule, Darmstadt 1969.

  • Wanner, H.: Enzyme der Glycolyse im Phloemsaft. Ber. schweiz. bot. Ges. 63, 200–212 (1953).

    Google Scholar 

  • Ziegler, H.: Untersuchungen über Leitung und Sekretion der Assimilate. Planta (Berlin) 47, 447–500 (1956).

    Google Scholar 

  • —, Ziegler, I.: Der Einfluß der Belichtung auf die NADP+-abhängige Glyceraldehyd-3-phosphat-Dehydrogenase. Planta (Berlin) 65, 369–380 (1965).

    Google Scholar 

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Authors and Affiliations

  1. Botanisches Institut der Technischen Hochschule Darmstadt, Darmstadt, Deutschland

    Mario Kennecke, Hubert Ziegler & Maria Alejandra Rongine de Fekete

  2. Botanisches Institut der Technischen Universität München, München, Deutschland

    Mario Kennecke, Hubert Ziegler & Maria Alejandra Rongine de Fekete

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  1. Mario Kennecke
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  2. Hubert Ziegler
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  3. Maria Alejandra Rongine de Fekete
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Kennecke, M., Ziegler, H. & de Fekete, M.A.R. Enzymaktivitäten im Siebröhrensaft von Robinia pseudoacacia L. und anderer Baumarten. Planta 98, 330–356 (1971). https://doi.org/10.1007/BF00380234

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