Summary
During pollen development and meiosis in Lilium henryi the protein pattern was analysed by disc electrophoresis, the microsporocytes and the tapetal fractions, being taken seperately. The pattern undergoes a phase-specific variation: every cytological stage shows a specific protein pattern.
The fractions could be partially identified by incubation with substrate and by specific enzyme staining of some of the enzymes (phosphorylase, acid phosphatase, peroxydase, thiamine pyrophosphatase, malate dehydrogenase, lactate dehydrogenase). The enzyme pattern also has a high phase specificity. Iso-enzymes were found in various numbers and activity, especially in the case of peroxydase and lactat dehydrogenase. The results are interpreted as a series of phase-specific gene transcriptions which coordinate the sequence of biochemical events essential for the normal pollen meiosis and the subsequent development of the pollen.
Zusammenfassung
Während der Pollenmeiose und Pollenentwicklung wurde, getrennt für die Mikrosporocyten- und Tapetumfraktion, mittels Disc-Elektrophorese das Proteinmuster ermittelt. Dieses zeigt eine phasenspezifische Veränderung, so daß jedem Stadium ein spezifisches Proteinmuster zugeordnet werden kann. Eine teilweise Identifizierung der Fraktionen wurde durch Direktfärbung einiger Enzyme (Phosphorylase, saure Phosphatase, Peroxydase, TPP, MDH und LDH) erzielt. Das Enzymmuster weist starke phasenspezifische Änderungen und eine größere Anzahl von Isoenzymen, vor allem bei der LDH und Peroxydase, auf.
Die Befunde werden als Serien von phasenspezifischen Gen-Transkriptionen gedeutet, welche den geordneten Ablauf der Meiose im Pollen und die Pollenreifung garantieren.
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Literatur
Allen, J.M.: The properties of golgi-associated nucleoside diphosphatase and thiamine pyrophosphatase: II. Electrophoretic separation and identification. J. Histochem. Cytochem. 11, 542–552 (1963).
— and J., Gockerman: Electrophoretic separation of multiple forms of particle associated acid phosphatase. Ann. N. Y. Acad. Sci. 121, 616–633 (1964).
Bingham, W.E., S.L., Krugman, and E.F. Estermann: Acrylamide electrophoresis of pine pollen protein. Nature (Lond.) 202, 923–924 (1964).
Cantino, E.C., and A. Goldstein: Protein changes during morphological differentiation and its reversal in synchronized single generations of Blastocladiella emersonii. Amer. J. Bot. 49, 642–646 (1962).
Carniel, K.: Das Antherentapetum. Öst. bot. Z. 110, 145–176 (1963).
Davis, B.J.: Disc electrophoresis — II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121, 404–427 (1964).
Desborough, S.L., and S.J. Peloquin: Disc electrophoresis of tuber proteins from Solanum species and interspecific hybrids. Amer. J. Bot. 52, 626 (1965).
Erickson, E.O.: Synchronous cell and nuclear division in tissues of higher plants. In: Synchrony in cell division and growth (ed. E. Zeuthen), p. 11–38 New York: Interscience 1964.
Foster, T.S., and H. Stern: The accumulation of soluble deoxyribosidic compounds in relation to nuclear division in anthers of Lilium longiflorum. J. biochem. biophys. Cytol. 5, 187–192 (1959).
Frederick, J.F.: An algal α-glucan phosphorylase which requires adenosine-5-phosphate as coenzyme. Phytochemistry 2, 413–415 (1963).
Hotta, Y., and H. Stern: Synthesis of messenger-like ribonucleic adic and protein during meiosis in isolated cells of Trillium erectum. J. Cell Biol. 19, 45–58 (1963).
Laufer, H.: Forms of enzymes in insect development. Ann. N. Y. Acad. Sci. 94, 825–835 (1961).
Linskens, H.F.: Physiologische Untersuchungen zur Reifeteilung. I. Über die Änderung einiger physiologischer Zustandsgrößen während der Pollenmeiose und Pollenentwicklung von Lilium henryi. Ber. dtsch. bot. Ges. 69, 353–360 (1956).
— II. Über die Änderung des Nukleinsäuregehaltes während der Pollenmeiose und Pollenentwicklung von Lilium henryi. Acta bot. neerl. 7, 61–68 (1958).
—, u. J.A.M. Schrauwen: III. Änderungen des Gehaltes an Sulfhydryl-Gruppen während der Pollenmeiose und Pollenentwicklung. Biol. Plant. (Praha) 5, 239–248 (1963).
Markert, C.L., and F. Møller: Multiple forms of enzymes: tissue, ontogenetic, and species specific patterns. Proc. nat. Acad. Sci. (Wash.) 45, 753–763 (1959).
McCune, D.C.: Multiple peroxydases in corn. Ann. N. Y. Acad. Sci. 94, 723–730 (1961).
Nasatir, M., A.M. Bryan, and S.D. Rodenberg: Changes in soluble proteins of developing lily anthers. Science 132, 897–898 (1960).
Neumann, K.: Meiotische Teilungswellen in Antheren und ihre morphologische Bedingtheit. Biol. Zbl. 82, 665–719 (1963).
Nygaard, A.P.: Various forms of d- and l-lactate dehydrogenase in yeast. Ann. N. Y. Acad. Sci. 94, 774–779 (1961).
Ornstein, L.: Disc electrophoresis — I. Background and theory. Ann. N. Y. Acad. Sci. 121, 321–349 (1964).
Raymond, S., and M. Nakamichi: Electrophoresis in synthetic gels, I and II. Analyt. Biochem. 3, 23–30 (1962); 7, 225–232 (1964).
—, and L. Weinstraub: Acrylamide gel as a supporting medium for zone electrophoresis. Science 130, 711 (1959).
Reisfeld, R.A., U.J. Lewis, and D.E. Williams: Disc electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature (Lond.) 195, 281–283 (1962).
Resende, F., H.F. Linskens, and F.M. Catarino: Growth regulators and protein content, their correlation with endopolyploidy. Rev. Biol. (Lisboa) 4, 101–112 (1964).
Rodrigues Pereira, A.S., and H.F. Linskens: The influence of glutathion and glutathione antagonists on meiosis in excised anthers of Lilium henryi. Acta bot. neerl. 12, 302–314 (1963).
Schrauwen, J.: Entfernung der Untergrund-Färbung nach Disc-Elektrophorese. J. Chromatogr. 15, 256–257 (1964).
Schrauwen, J. Nachweis von Enzymen nach elektrophoretischer Trennung an Polyacrylamid-Säulchen. J. Chromatogr. 22 (in press) (1966).
Spiegelman, S.: Information transfer from the genom. Fed. Proc. 22, 36–54 (1963).
Taylor, J.H.: Autoradiographic studies of nucleic acids and proteins during meiosis in Lilium longiflorum. Amer. J. Bot. 46, 477–484 (1959).
Vasil, I.K.: Physiology of anthers. Proc. Summer School of Botany-Darjeeling (ed. P. Maheshwari, B.M. Johri and I.K. Vasil), p. 477–487. Goverment of India: New Delhi 1962.
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Linskens, H.F. Die Änderung des Protein- und Enzym-Musters Während der Pollenmeiose und Pollenentwicklung. Planta 69, 79–91 (1966). https://doi.org/10.1007/BF00380213
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DOI: https://doi.org/10.1007/BF00380213