Abstract
A cell line of M. polymorpha was grown photoautotrophically in liquid suspension culture using 1% CO2 in air as sole carbon source. The growth rate in terms of cell dry-weight during the exponential phase was 0.171 and the doubling time was 1.76 d. The rate of increase in chlorophyll was 1.6 times higher than the growth rate. The highest content of chlorophyll was 24 mg g-1 dry weight, and the photosynthetic activity of the cells in the exponential phase, as calculated from the growth rate, was at least 60 μmol mg-1 chlorophyll h-1.
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References
Berlyn, M.B., Zelitch, I.: Photoautotrophic growth and photosynthesis in tobacco callus cells. Plant Physiol. 56, 753–756 (1975)
Berlyn, M.B., Zelitch, I., Beaudette, P.B.: Photosynthetic characteristics of photoautotrophically grown tobacco callus cells. Plant Physiol. 61, 606–610 (1978)
Hüsemann, W., Barz, W.: Photoautotrophic growth and photosynthesis in cell suspension culture of Chenopodium rubrum. Physiol. Plant. 40, 77–81 (1977)
Iwamura, T., Nagai, H., Ichimura, S.: Improved methods for determining contents of chlorophyll, protein, ribonucleic acid, and deoxyribonucleic acid in planktonic populations. Int. Res. Ges. Hydrobiol. 55, 131–147 (1970)
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497 (1962)
Ohta, Y., Katoh, K., Miyake, K.: Establishment and growth characteristics of a cell suspension culture of Marchantia polymorpha L. with high chlorophyll content. Planta 136, 229–232 (1977)
Takabayashi, A., Nishimura, T., Iwamura, T.: Incorporation of radioactive tracers of some labelled compounds into DNA, RNA, and protein in Chlorella cells at various stages of the cell cycle in synchronous culture. J. Gen. Appl. Microbiol. 22, 183–196 (1976)
Tamiya, H., Iwamura, T., Shibata, K., Hase, E., Nihei, T.: Correlation between photosynthesis and light-independent metabolism in the growth of Chlorella. Biochem. Biophys. Acta 12, 23–40 (1953)
Yamada, Y., Sato, F.: The photoautotrophic culture of chlorophyllous cells. Plant Cell Physiol. 19, 691–699 (1978)
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Katoh, K., Ohta, Y., Hirose, Y. et al. Photoautotrophic growth of Marchantia polymorpha L. cells in suspension culture. Planta 144, 509–510 (1979). https://doi.org/10.1007/BF00380131
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DOI: https://doi.org/10.1007/BF00380131