Abstract
Isolated chromoplasts from Narcissus pseudonarcissus flowers contain: a fatty acid synthesizing system; acyl-CoA synthetase (EC 6.2.1.3); glycero-phosphate acyltransferase (EC 2.3.1.15); acylglycero-phosphate acyltransferase; phosphatidate phosphatase (EC 3.1.3.4); diacylglycerol galactosyltransferase (EC 2.4.1.46); and diacylgalactosylglycerol galactosyltransferase, i.e. all enzymatic activities necessary for the synthesis of diacylgalactosylglycerol and diacylgalabiosylglycerol from acetate, HCO 3- , sn-glycerol 3-phosphate, and UDP-d-galactose. Diacylgalactosylglycerol and diacylgalabiosylglycerol, however, are synthesized from these precursors to only a very low extent in an in vitro system. This is attributed to a specificity of diacylglycerol galactosyltransferase for highly unsaturated diacylglycerols. Specificities of acyltransferase reactions were also found.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bertrams, M., Heinz, E.: Experiments on enzymatic acylation of sn-glycerol 3-phosphate with enzyme preparations from pea and spinach leaves. Planta 132, 161–168 (1976)
Bolton, P., Harwood, J.L.: Fatty acid synthesis by slices from developing leaves. Planta 138, 223–228 (1978)
Douce, R.: Sites of biosynthesis of galactolipids in spinach chloroplasts. Science 183, 852–853 (1974)
Douce, R., Guillot-Salomon, T.: Sur l'incorporation de la radioactivité du sn-glycerol-3-phosphate-14C dans le monogalactosyl-diacylglyceride des plastes isolés. FEBS Lett. 11, 121–124 (1970)
Eccleshall, T.R., Hawke, J.C.: Biosynthesis of monogalactosyl diglyceride by chloroplasts from Spinacia oleracea and from some gramineae. Phytochemistry 10, 3035–3045 (1971)
Heinz, E., Harwood, J.L.: Incorporation of carbon dioxide, acetate and sulphate into the glycerolipids of Vicia faba leaves. Hoppe-Seyler's Z. Physiol. Chem. 358, 897–908 (1977)
Heinz, E., Bertrams, M., Joyard, J., Douce, R.: Demonstration of an acyltransferase activity in chloroplast envelopes. Z. Pflanzenphysiol. 87, 325–331 (1978)
Joyard, J., Douce, R.: Mise en evidence et role des diacylglycerols de l'envelope des chloroplastes d'épinard. Biochim. Biophys. Acta 424, 125–131 (1976)
Joyard, J., Douce, R.: Site of synthesis of phosphatidic acid and diacylglycerol in spinach chloroplasts. Biochim. Biophys. Acta 486, 273–285 (1977)
Kannangara, C.G., Stumpf, P.K.: Fat metabolism in higher plants. L. The biosynthesis of polyunsaturated fatty acids by isolated spinach chloroplasts. Arch. Biochem. Biophys. 148, 414–424 (1972)
Kleinig, H., Liedvogel, B.: Fatty acid synthesis by isolated chromoplasts from the daffodil. [14C]acetate incorporation and distribution of labelled acids. Eur. J. Biochem. 83, 499–505 (1978)
Kleinig, H., Liedvogel, B.: Fatty acid and glycerolipid synthesis in abscised daffodil flowers after acetate feeding. Planta 144 473–477 (1979)
Leech, R.M., Rumsby, M.G., Thomson, W.W.: Plastid differentiation, acyl lipid and fatty acid changes in developing green maize leaves. Plant Physiol. 52, 240–245 (1973)
Liedvogel, B., Kleinig, H.: Galactolipid synthesis in chromoplast internal membranes of the daffodil. Planta 129, 19–21 (1976)
Liedvogel, B., Kleinig, H.: Lipid metabolism in chromoplast membranes from the daffodil: glycosylation and acylation. Planta 133, 249–253 (1977)
Liedvogel, B., Kleinig, H., Thompson, J.A., Falk, H.: Chromoplasts of Tropaeolum majus L.: lipid synthesis in whole organelles and subfractions. Planta 141, 303–309 (1978)
Mudd, J.B., Garcia, R.E.: Biosynthesis of glycolipids, In: Recent Advances in the Chemistry and Biochemistry of Plant Lipids, pp. 161–201, Galliard, T., Mercer, E., eds. London, New York, San Francisco: Academic Press 1975
Mudd, J.B., van Vliet, H.H.D.M., van Deenen, L.L.M.: Biosynthesis of galactolipids by enzyme preparations from spinach leaves. J. Lipid Res. 10, 623–630 (1969)
Murphy, D.J., Leech, R.M.: Lipid biosynthesis from [14C]bicarbonate, [2-14C]pyruvate and [1-14C]acetate during photosynthesis by isolated spinach chloroplasts. FEBS Lett. 77, 164–168 (1977)
Ongun, A., Mudd, J.B.: Biosynthesis of galactolipids in plants. J. Biol. Chem. 243, 1558–1566 (1968)
Roughan, P.G., Slack, C.R.: Long-chain acyl-coenzyme A synthetase activity of spinach chloroplasts is concentrated in the envelope. Biochem. J. 162, 457–459 (1977)
Shine, W.E., Mancha, M., Stumpf, P.K.: Fat metabolism in higher plants. Differential incorporation of acyl-coenzymes A and acyl-acyl carrier proteins into plant microsomal lipids. Arch. Biochem. 173, 472–479 (1976)
Slack, C.R., Roughan, P.G.: The kinetics of incorporation in vivo of [14C]acetate and [14C]carbon dioxide into the fatty acids of glycerolipids in developing leaves. Biochim. J. 152, 217–228 (1975)
Van Besouw, A., Wintermanns, J.F.G.M.: Galactolipid formation in chloroplast envelopes. I. Evidence for two mechanisms in galactosylation. Biochim. Biophys. Acta 529, 44–53 (1978)
Van Hummel, H.C., Hulsebos, T.J.M., Wintermanns, J.F.G.M.: Biosynthesis of galactosyl diglycerides by non-green fractions from chloroplasts. Biochim. Biophys. Acta 380, 219–226 (1975)
Williams, J.P., Watson, G.R., Leung, S.P.K.: Galactolipid synthesis in Vicia faba leaves. II. Formation and desaturation of long chain fatty acids in phosphatidylcholine, phosphatidylglycerol, and the galactolipids. Plant Physiol. 57, 179–184 (1976)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liedvogel, B., Kleinig, H. Galactolipid synthesis in chromoplasts in vitro. Planta 144, 467–471 (1979). https://doi.org/10.1007/BF00380124
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00380124